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ÎÒÄÅË ÝÍÇÈÌÎËÎÃÈÈ

ÏÀÂÅË ÏÀÂËÎÂÈ× ÔÈËÈÏÏÎÂ

çàâåäóþùèé îòäåëîì, äîêòîð áèîëîãè÷åñêèõ íàóê, ïðîôåññîð; ëàóðåàò ïðåìèè èì. Þ.À.Îâ÷èííèêîâà Ðîññèéñêîé Àêàäåìèè íàóê (1997 ãîä)

"Ìîëåêóëÿðíûå ìåõàíèçìû çðåíèÿ" (ðóêîâîäèòåëè: ïðîô. Ï.Ï.Ôèëèïïîâ, ê.õ.í. È.È.Ñåíèí). Íàèáîëåå çíà÷èòåëüíûé ðåçóëüòàò ïî ýòîìó íàïðàâëåíèþ - îáíàðóæåíèå ôîòîðåöåïòîðíîãî Ñà2+-ñâÿçûâàþùåãî áåëêà ðåêîâåðèíà è óñòàíîâëåíèå åãî ôóíêöèè.  íàñòîÿùåå âðåìÿ îñíîâíîé îáúåêò èññëåäîâàíèé - ñèñòåìà ðîäîïñèí - ðîäîïñèíêèíàçà - ðåêîâåðèí, ðåêîíñòðóèðîâàííàÿ èç ïðèðîäíûõ è ðåêîìáèíàíòíûõ áåëêîâ.

"Ìîëåêóëÿðíûå ìåõàíèçìû íåéðîäåãåíåðàòèâíûõ ïðîöåññîâ ïðè çëîêà÷åñòâåííûõ íîâîîáðàçîâàíèÿõ è ðàííÿÿ äèàãíîñòèêà ðàêà" (ðóêîâîäèòåëè: ïðîô. Ï.Ï.Ôèëèïïîâ, ê.õ.í. È.È.Ñåíèí, ê.á.í. Í.Ê.Òèõîìèðîâà). Êëåòêè îäíîãî èç íàèáîëåå òÿæåëûõ è ðàñïðîñòðàíåííûõ âèäîâ ðàêà - ìåëêîêëåòî÷íîé êàðöèíîìû ëåãêèõ (SCLC) - ñïîñîáíû ñèíòåçèðîâàòü ðåêîâåðèí, êîòîðûé â íîðìå ïðèñóòñòâóåò òîëüêî â ñåò÷àòêå ãëàçà. Êàê ñëåäñòâèå, â êðîâè ïàöèåíòîâ óæå íà ðàííèõ ñòàäèÿõ ðàêà ìîãóò ïîÿâëÿòüñÿ àíòèòåëà ê ðåêîâåðèíó, âûçûâàþùèå ó íèõ íàðóøåíèÿ çðåíèÿ - ðåòèíîïàòèþ. Ðàáîòà âåäåòñÿ ñ öåëüþ âûÿñíåíèÿ ìîëåêóëÿðíûõ ìåõàíèçìîâ SCLC-àññîöèèðîâàííîé ðåòèíîïàòèè è ñîçäàíèÿ ìåòîäîâ ðàííåé äèàãíîñòèêè ðàêà íà îñíîâå ðåêîâåðèíà è àíòèòåë ê íåìó. Åùå îäèí ïîòåíöèàëüíûé ìàðêåð ðàêîâûõ çàáîëåâàíèé - Hu-àíòèãåí - òàêæå íàõîäèòñÿ â ñôåðå èíòåðåñîâ îòäåëà.

"Ðåöåïöèÿ ñòåðîèäíûõ ãîðìîíîâ" (ðóêîâîäèòåëü: ê.á.í. Ë.Ë.Èãíàòåíêî). Èññëåäîâàíèÿ ïî ýòîìó íàïðàâëåíèþ âåäóòñÿ ñ öåëüþ âûÿñíåíèÿ ìîëåêóëÿðíûõ ìåõàíèçìîâ òðàíñìåìáðàííîé ïåðåäà÷è ñèãíàëîâ ÷åðåç ðåöåïòîðû ñòåðîèäíûõ ãîðìîíîâ è ðàçðàáîòêè òåñò-ñèñòåìû äëÿ îïðåäåëåíèÿ ñòàòóñà ýòèõ ãîðìîíîâ ïðè áîëåçíÿõ (â òîì ÷èñëå îïóõîëåâûõ) æåíñêîé ïîëîâîé ñôåðû.

Èçáðàííûå íîâûå ðàáîòû

1. Dizhoor A.M., Ray S., Kumar S., Niemi G., Spencer M., Brolley D., Walsh K.A., Philipov P.P., Hurley J.B., Stryer L. Recoverin: a calcium sensitive activator of retinal rod guanylate cyclase Science (1991) 251, 915-918.

2. Ray S., Zozulya S., Niemi G.A., Flaherty K.M., Brolley D., Dizhoor A.M., McKay D.B., Hurley J., Stryer L. Cloning, expression, and crystallization of recoverin, a calcium sensor in vision. Proc. Natl. Acad. Sci. USA (1992) 89, 5705-5709.

3. Dizhoor A.M., Ericsson L.H., Johnson R.S., Kumar S., Olshevskaya E., Zozulya S., Neubert T.A., Stryer L., Hurley J.B., Walsh K.A. The NH2 terminus of retinal recoverin is acylated by a small family of fatty acids. J. Biol. Chem. (1992) 267, 16033-16036.

4. Dizhoor A.M., Chen C.K., Olshevskaya E., Sinelnikova V.V., Phillipov P., Hurley J.B. Role of the acylated amino terminus of recoverin in Ca(2+)-dependent membrane interaction. Science (1993) 259, 829-832.

5. Senin I.I., Dean K.R., Zargarov A.A., Akhtar M., Philippov P.P. Recoverin inhibits the phosphorylation of dark-adapted rhodopsin more than it does that of bleached rhodopsin: a possible mechanism through which rhodopsin kinase is prevented from participation in a side reaction.Biochem. J. (1997) 321, 551-555.