... 22350 22356, 2003 Printed in U.S.A. Distinctive Properties of the 5 -Untranslated Region of Human Hsp70 mRNA * Received for publication, March 28, 2003 Published, JBC Papers in Press, April 7, 2003, DOI 10.1074/jbc.M303213200 Maria P. Rubtsova ... the §Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 117984, Russia A relaxed cap-dependence of translation of the mRNA -encoding mammalian heat shock protein ... IRES-properties of the 5 -UTR of Hsp70 mRNA 22353 FIG....
... 8 A Leaderless mRNA Can Bind to Mammalian 80S Ribosomes and Direct Polypeptide Synthesis in the Absence of Translation Initiation Factors Dmitri E. Andreev, Ilya M. Terenin, Yan E. Dunaevsky, Sergei E. Dmitriev, and Ivan N. Shatsky* Belozersky Insitute of Physico-Chemical Biology, Moscow State University, Bldg. ... Thus, the 80ScIlacZ mRNAMet-tRNAMet complex i formed without translation initiation factors was totally competent for the elongation step of polypeptide synthesis. ...
Downloaded from www.rnajournal.org on January 28, 2008 - Published by Cold Spring Harbor Laboratory Press The bacterial toxin RelE induces specific mRNA cleavage in the A site of the eukaryote ribosome Dmitri Andreev, Vasili Hauryliuk, Ilya Terenin, Sergey Dmitriev, MÅns Ehrenberg and Ivan Shatsky RNA 2008 14: 233-239; originally ... This could mean that although RelE could cleave mRNA in the 48S initiation complex, the toxin was unable cleave in the eukaryotic 80S ribosome. ... Fig. ...
Downloaded from www.rnajournal.org on September 7, 2007 Differential factor requirement to assemble translation initiation complexes at the alternative start codons of foot-and-mouth disease virus RNA Dmitri E. Andreev, Olga Fernandez-Miragall, Jorge Ramajo, Sergey E. Dmitriev, Ilya M. Terenin, Encarna Martinez-Salas and Ivan N. Shatsky RNA 2007 13: 1366-1374; originally published online Jun 25, 2007; Access the most recent version at doi:10.1261/ ... Copyright ñ 2007 RNA Society. ...
... The extremely long and GC-rich L1 5 untranslated region (5 UTR) directs synthesis of numerous copies of RNA-binding protein ORF1p per mRNA. ... 27, 2007 LINE-1 mRNA TRANSLATION INITIATION 4687 Downloaded from mcb.asm.org at UNIV OF MIAMI SCH OF MED on June 18, 2007 FIG. ... As shown in Fig. ... The capped L1 5 UTR-Fluc mRNA was translated four to five times more effectively than the uncapped HRV-Fluc mRNA, where the translation of Fluc was directed by a rather strong picornavirus IRES (Fig. ...
... Recently, the 579-nucleotidelong 5 untranslated region (UTR) of RhPV has been shown to contain an internal ribosome entry site (IRES) that functions efficiently in mammalian, plant, and insect in vitro translation systems. Here, the mechanism of action of the RhPV IRES has been characterized by reconstitution of mammalian 48S initiation complexes on the IRES from purified components combined with the toeprint assay. ... Purification of 40S ribosomal subunits, initiation factors, and Met-tRNAiMet. ...
... FT (Feature Table), ...; elements of description fields are referred to as words. ... P (1) 1 =N , P n q (2) = n N- n , P all q 2 ( +) 2 = N (n n + all is the total number of words for which the prediction is performed, i.e., total number of KW-type words in description fields of all sequences , similar to at least one query sequence , nq is the number of words (from the list of these nall words ) that belong to query sequences , n+ is the total number of words for which the ...
... PDF doc] An automatic annotation of a sequence is based on statistics assembled from the result of the homology search, that is for a prediction description elements (DEL) of the given sequence. ... The desired percent of coinciding (from the maximum in the case of complete similarity) "one-color" pair-patterns on the selected shift (in 1/100 of 1) (0.02 for protein and 0.08 for nucleotide query sequence). ... Title: Amino acid substitution matrices from protein blocks. ...
IGLA-3D is a modular method for construction of pairwise 3D alignments (superpositions) of protein structures. ... the first module (IS) constructs an initial superposition of two protein structures or an initial core, the second module (AC) constructs alignment core given proteins superposition, the third module (FM) finds a motion (shift and rotation) that optimally superimposes the alignment core, the forth module (SM) evaluates the similarity of proteins given a 3D alignment of these proteins. ...
... 2.9 Using a pre-computed guide tree . ... 3 File Formats . ... With challenging sets of sequences, it is a good idea to make several different alignments using different muscle options and to try other programs too. ... You can ask muscle to try to improve an existing alignment by using the – refine option. ... If you have two existing alignments of related sequences you can use the –profile option of MUSCLE to align those two sequences. ... MUSCLE uses FASTA format for both input and output. ...