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Электронная библиотека Попечительского совета механико-математического факультета Московского государственного университета
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Balkwill F. (ed.) - Cytokine Molecular Biology |
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Предметный указатель |
5' Nuclease assay 36-38
Acidic Smase 150
Allelic discrimination methods 25-29
Amino-acid sequence analysis 81-84
Antibodies to study cell, signalling 107-108 112-115 119 122
Antisense asymmetric RNA probes 63-64
Association studies for disease-causing alleles 40-43
Auto-Kinase assay 116-117
Autographa californica MNPV vector 11
Automated protein sequences 81-82
Autoradiography in situ hybridisation 70-71
BLAST program 9
Blastocyst injection 176-180
Bradford assay 110
Case control studies for gene polymorphism 23-25 40-41
cDNA assays 48
cDNA libraries 1-20
cDNA synthesis 2-3 16 58-59
Cell signalling see receptor signalling
Ceramide 134 136-137
Charring densitometry 146-148
Chemical synthesis of chemokines see peptide synthesis
Chemokines - purification 73-88
Chimaeric mice 155-188
CHO cells 11
Cloning of cytokine genes 1-20
Cloning of cytokine genes, IFN- receptors 100-101
Cloning of cytokine genes, IFN-7 receptor 98-100
Conditional gene targeting 180-186
Contamination in PCR-based techniques 38-39
COS cells 10
Covalent crosslinking of ligand to receptor 96-97
CPG 75-79
Cre recombinase 180-186
Cre-expressing transgenic lines 183-186
Crosslinking of ligand to receptor 96-97
Cysteine residue modification 84-85
Cytosolic extracts 126-127
Dag 134 136-137
DNase treatment of RNA 51-53
Egg transfer for transgenic mice 160-161
Electrotransformation of E. coli 5
Embryonic stem cells 153 88
EMSA 125-130
ES cells see embryonic stem cells
EST databases 9
Expression of cytokine genes 1-20
Expression of cytokine genes, bacterial systems 5 7 14-16
Expression of cytokine genes, insect cells 11
Expression of cytokine genes, lower eukaryotes 12-14
Expression of cytokine genes, mammalian cells 7
Expression of cytokine genes, permanent expression 10-11
Expression of cytokine genes, transient expression 10
Expression vectors 4 10-16
Fluorescence-based allelic, discrimination 35-36
G proteins 105-107
GAPDH control 52
Gel retardation assay see EMSA
Genetic variation of cytokine loci see polymorphisms of cytokine genes
Genomic DNA preparation 27-28
Haplotype relative-risk analysis 41
HEK293T cells 10
Immunoprecipitation 114-115
In situ hybridisation to cytokine mRNA 62-72
In situ hybridisation to cytokine mRNA, antisense probes 65
In situ hybridisation to cytokine mRNA, autoradiography 70-71
In situ hybridisation to cytokine mRNA, controls 64-65
In situ hybridisation to cytokine mRNA, counterstaining 71-72
In situ hybridisation to cytokine mRNA, dark field microscopy 71-72
In situ hybridisation to cytokine mRNA, DNA probes 67-68
In situ hybridisation to cytokine mRNA, hybridisation 68-69
In situ hybridisation to cytokine mRNA, Interpretation 71-72
In situ hybridisation to cytokine mRNA, posthybridisation 69-70
In situ hybridisation to cytokine mRNA, prehybridisation 63 66-67
In situ hybridisation to cytokine mRNA, probe choice 63-64
In situ hybridisation to cytokine mRNA, probe labelling 67-68
In situ hybridisation to cytokine mRNA, sample handling 63
In situ hybridisation to cytokine mRNA, sample preparation 66-67
In situ hybridisation to cytokine mRNA, sense probes 65
In situ hybridisation to cytokine mRNA, slide preparation 65
In vitro kinase assay 115-116 112-113
Inflammatory diseases 21-46
Interferon- cloning 14
Interferon- , control for northern blotting 52
Interferon- , expression 11
Interleukin-2(IL-2), cloning 13 14
Interleukin-2(IL-2), control for northern blotting 52
Interleukin-4(IL-4) cloning 14
Interleukin-5(IL-5) expression 13
Interleukin-6(IL-6), control for northern blotting 52
Interleukin-6(IL-6), expression 11 13-14
Interleukin-l(IL-l), control for northern blotting 52
Interleukin-l(IL-l), genetic polymoprohisms 21-46
Internal sequence analysis 83-84
Jak kinases 105 114-115
Knockout mice 166-188
Knockout mice, blastocyst injection 176-180
Knockout mice, chimaeric mice generation 179
Knockout mice, conditional gene targeting 180-186
Knockout mice, electroporation of DNA 171-172
Knockout mice, embryonic stem (ES) cells 167-168
Knockout mice, extraction of DNA 175-176
Knockout mice, feeder cell layers 168-169
Knockout mice, freezing ESC clones 173-174
Knockout mice, gene targeting ES 169-171
Knockout mice, germline chimeras 176-180
Knockout mice, lacZ assays 184-186
Knockout mice, picking and expansion of ESC cells 172-173
Knockout mice, storage and recovery of ESC cells 174-175
LacZ activity 184-188
Lipid extraction 140-141
Lipidkinases 105-107
Lysis of cells 109-111
MAPK pathway activity 119-123
Metabolic cell labelling 139-140
MHC and immune response 21 23
Microinjection 156-160
MlP- 73
Neutral Smase 149
Non-parametric linkage analysis 42-43
Northern analysis of cytokine mRNA 52-58
Northern analysis of cytokine mRNA, blotting 54-55
Northern analysis of cytokine mRNA, electrophoresis 53-54
| Northern analysis of cytokine mRNA, handling RNA 48-49
Northern analysis of cytokine mRNA, hybridisation 58-58
Northern analysis of cytokine mRNA, isolation of RNA 49-51
Northern analysis of cytokine mRNA, preparation of RNA 48-49
Northern analysis of cytokine mRNA, probe labelling 55-56
Nuclear protein extracts 126-127
Oligonucleotide screening 8
Oocyte expression of cytokines 5-6
Oocyte recovery 158-159
PC-specific phosphlipase C 133-153
PCR for hybridisation probes 9
PCR reaction 59-61
PCR-grade DNA preparation 26-27
PCR-RFLPgenotyping 29-34
Peptide synthesis 85-88
Peptide synthesis, solid phase 87-88
Percill preparation, phospholipase D 133-154
Phosphatidyl choline 133-154
Phosphatidylcholine-specific, phospholipase C 133-154
Phosphotyrosine western blotting 112-115
Pkhiapastoris 13
Polymerase chain reaction 28-29
Polymerase chain reaction for cytokine mRNA 58-61
Polymerase chain reaction for cytokine mRNA, cDNA synthesis 58-59
Polymerase chain reaction for cytokine mRNA, cycle parameters 50-61
Polymerase chain reaction for cytokine mRNA, primers 60
Polymerase chain reaction for cytokine mRNA, reaction 59-60
Polymerase chain reaction for cytokine mRNA, verification 61
Polymorphisms of cytokine genes 21-46
Polymorphisms of cytokine genes, case control studies for TNF and IL-1 gene polymophism 23-25
Polymorphisms of cytokine genes, genetic variation at IL-1 and TNF loci 21-23
Polymorphisms of cytokine genes, methods for allelic discrimination in IL-1 and TNF gene clusters 25-26
Polymorphisms of cytokine genes, quality controls 38-39
Polymorphisms of cytokine genes, screening 30-36
Polymorphisms of cytokine genes, study design 39-43
Primers for cytokine RT-PCR 60
Protein fragmentation for internal sequence analysis 83-84
Protein purification 73-88
Purification of cytokines and chemokines 73-88
Purification of cytokines and chemokines, adsorption to CPG 75-80
Purification of cytokines and chemokines, adsorption to silicic acid 79-81
Purification of cytokines and chemokines, purification of chemokines from natural sources 75-81
Purification of cytokines and chemokines, sequence analysis 81-85
Radiolabelling, consensus oligonucleotides 127-129
Radiolabelling, cytokines 90-92
Radiolabelling, probes 55-56
RANTES 73
Receptor signalling 105-153
Receptor signalling, acidic Smase 150
Receptor signalling, auto-kinase assay 116-117
Receptor signalling, ceramide 134 136-137
Receptor signalling, charring densitometry 146-148
Receptor signalling, cytostolic extracts 126-127
Receptor signalling, DAG 134 138-143
Receptor signalling, determination of cell lysate protein concentration 111
Receptor signalling, EMSA 125-126 129-130
Receptor signalling, inhibitors 108
Receptor signalling, JAK kinase activity 114-115
Receptor signalling, lipid extraction 140-141
Receptor signalling, MAPK pathway activity 119-120
Receptor signalling, metabolic cell labelling 139-140
Receptor signalling, neutral Smase 149
Receptor signalling, nuclear protein extracts 126-127
Receptor signalling, PC-specific phospholipase C 133-153
Receptor signalling, phosphotyrosine western blotting 112-114
Receptor signalling, preparation of cells for signalling studies 108-111
Receptor signalling, radiolabelling consensus oligonucleotides 127-129
Receptor signalling, serine-threonine kinases 118-119
Receptor signalling, sphingomyelinases 133-153
Receptor signalling, Src kinase assays 118
Receptor signalling, thin-layer chromatography for detection of DAG 141-143
Receptor signalling, thin-layer chromatography for detection of Pchol 143-145
Receptor signalling, transcription factor assay 123-124
Receptor signalling, tyrosine phosphorylation and tyrosine kinases 111-114
Receptor-binding studies 89-104
Receptor-binding studies, binding of ligand 93-95
Receptor-binding studies, covalent crosslinking 96-97
Receptor-binding studies, radiolabelling interferon 91-92
Receptor-binding studies, scatchard analysis 94
Ribonuclease protection assay 61-62
RNA 1-4 47-72
RNA, conversion to cDNA 2-3
RNA, in situ hybridisation 62-72
RNA, Northern blotting 51-57
RNA, preparation 1-2 48-52
RNA, ribonuclease protection assay 61-62
RT-PCR for cytokine mRNA 58-61
Saccharomyces cerevisiae secretion system 12
Scatchard analysis 94
Serine/threonine kinases 118-119
Signalling from cytokine receptors see receptor Signalling
Silicic acid 75-76
Slot blots of tail DNA 163-164
Solid-phase peptide synthesis 87-88
Sphingomyelinases 133-153
Spodopterafrugiperda-derived cell lines 11
Src kinases 105 118
STAT transcription factors 124-130
Syk kinases 105
TagMan allelic discrimination 36-38
Tail DNA preparation 162-163
Tec kinases 105
Thin layer chromatography 141-145
TRADD 105
TRAFs 105
Transcription factors 105-107 123-130
Transgenic mice 155-188
Transgenic mice, animals 155-156
Transgenic mice, egg transfer 160-161
Transgenic mice, integration / expression transgenes 161-162
Transgenic mice, micromjection 159-160
Transgenic mice, PCR screening 165
Transgenic mice, pronuclear injection of DNA 157-158
Transgenic mice, purification of DNA 156-157
Transgenic mice, recovery of oocytes 158-159
Transgenic mice, slot blots of tail DNA 163-164
Transgenic mice, tail DNA preparation 162-163
Transmission/disequilibrium tests (TDT) 41-43
Tumour necrosis factors (TNFs), genetic polymorphism 21-46
Tumour necrosis factors (TNFs), signalling pathways 105-132
Tyrosine kinases 105-131
Tyrosine phosphorylation 105-131
Western blotting 112-114 119-123
Xenopus laevis oocytes 5-6 90 94-95
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