Human Life Research  on ISS Russian Segment
 	 CRISP-2 Experiment
 
	 
	 
 
	  Experiment CRISP-2 forms part of the scientific   research program performed by Italian Astronaut Roberto   Vittori (ESA) 
        on the Russian Segment of the ISS in   the frame of ENEIDE  ISM Project. 
         The European Space Agency is a customer    of the preparation and implementation of the research   program.
		 
   	  
 	
 		    Objective:
  
            -  	Study the effects of microgravity on the     proliferation of neurons in crickets, if fertilization  occurs in space.
 
            -  Study the effects of gravity deprivation  on gravity-related behavior in crickets.
 
    
   
   						  
	         Task:
 
		    Incubation of animals on the Russian Segment of   the ISS.
    
       
	 Science Hardware  Used:
						  - Experimental Cricket Containers CRI (2) with laboratory crickets, i.e. female crickets Acheta  domesticus, seven female crickets in each container.  Each Cricket Container accommodates three Egg Collectors   and one water bottle.
 
						   - KUBIK - AMBER - thermally   insulated container (incubator) with a set of   slots for Cricket Containers.
 
					 
					 
							 	 During flight, females will be allowed to deposit eggs in the   respective egg collectors during two periods of the mission.
                  This requirement considers that embryos are exposed for different  periods to microgravity. After the first period of eggs deposition
                  the embryos are developed for 8 days under microgravity conditions.   During this period the neuronal proliferation under Earth conditions
                  is completed. After the second period of egg deposition the  embryos remain in microgravity for 3 days only, namely, neuronal
                  proliferation will continue after return to 1g-condition. 
	
						
					    Support Facilities:
                          
						  
                        Consumables:
                        
			                       
					 Experiment Results:
                 
                 
                            - Incubated embryos of crickets in Cricket Containers;
 
                            - Video   data recorded during the experiment showing walking  abilities and health conditions of the female  crickets.