Tetrahedron Letters : A Novel, Efficient Regenerating Method of NADPH Using a New Formate Dehydrogenase

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Tetrahedron Letters

Volume 37, Issue 9		Abstract
26 February 1996		Journal Format-PDF (178 K)
Pages 1377-1380

0040-4039(96)00010-X

A Novel, Efficient Regenerating Method of NADPH Using a New Formate Dehydrogenase

Karsten Seelbach¹, Bettina Riebel², Werner Hummel², Maria-Regina Kula², Vladimir I. Tishkov³, Alexey M. Egorov³, Christian Wandrey¹ and Udo Kragl¹

¹ Forschungszentrum JÝlich GmbH, Institut fÝr Biotechnologie D-52425 JÝlich Germany
² Institut fÝr Enzymtechnologie, Heinrich Heine UniversitÄt, Forschungszentrum JÝlich GmbH, Postfach 2050 D-52404 JÝlich Germany
³ Department of Chemical Enzymology, Chemistry Faculty, Lomonosov Moscow State University 119899 Moscow Russia

Received 28 November 1995; accepted 21 December 1995. Available online 24 June 1999.

Abstract

A NADPH regenerating system using a new, protein engineered formate dehydrogenase (FDH) is investigated. The new enzyme is the first known NAD(P)H dependent FDH. It can be successfully employed in synthesis with other enzymes requiring a NADPH regeneration. All advantages of the known NAD(H) dependent FDH's in these enzyme coupled synthesis can now be transferred to NADP(H) dependent systems.

Tetrahedron Letters		Abstract Journal Format-PDF (178 K)
Volume 37, Issue 9
26 February 1996
Pages 1377-1380