DEPARTMENT OF PHOTOSYNTHESIS AND FLUORESCENT
METHODS OF INVESTIGATION
Andrei P. Razjivin
Head of Department, Sc.D.
Department was
organized in 1976. Prof. A.Yu. Borisov was the first Head of Department.
Since 1996 to present day Prof. A.P Razjivin is at the head.
Department leading researchers: Prof. A.Yu. Borisov, Prof. V.I. Novoderezhkin,
PhD E.A. Kotova, PhD A.S. Taisova.
Main lines of investigation: Theoretical and experimental studies
of primary processes of photosynthesis. Further development and application
of optical spectroscopy methods (derivative spectroscopy, fluorescence spectroscopy)
in Life Sciences.
Participation in research projects (programs) and grant support. Grants of Russian Foundation of Basic Research: "Interrelation
of spectral and structural characteristics of photosynthetic antennae according
experimental and theoretical investigations" (A.P.Razjivin, 2003-2005);
"Excitation transfer in pigment-protein complexes of photosynthesis:
experimental investigations by non-linear spectroscopy and theoretical simulation" (A.Yu. Borisov, 2005-2007);
Russian-Dutch grant RFBR-NWO "Mathematical modeling of energy transfer in
photosynthesis" (V.I. Novoderezhkin, 2004-2006), Interdisciplinary project
of
Major research advances: Demonstration of picosecond-scale processes of energy transfer
from light-harvesting chlorophyll to reaction centers in purple and green
bacteria and plant photosystem I [Borisov AY, Godik VI. Fluorescence lifetime
of bacteriochlorophyll and reaction center photooxidation in purple bacterium.
Biochim Biophys Acta. 1970; 221: 441-3; Borisov AY, Godik VI. Energy transfer
in bacterial photosynthesis. I. Light intensity dependences of fluorescence
lifetimes. Bioenergetics. 1972; 3: 211-20; Borisov AY, Il'ina MD. Fluorescence
lifetime and energy migration mechanism in photosystem-1 of plants. Biochim
Biophys Acta. 1973; 305: 364-73; Borisov AY, Fetisova ZG, Godik VI. Energy
transfer in photoactive complexes obtained from green bacterium
Awards: I.Shuvalov's avard of
Primary research publications:
1. Godik VI, Borisov AY. Excitation trapping by different states
of reaction centers. FEBS Lett. 1977; 82: 355-8.
2. Fok MV Borisov AY. Water and energy conversion in photosynthesis.
Studia Biophysica. 1981; 84: 115-124.
3. Borisov AY, Sidorin YM. The concept of energy migration and trapping
in purple bacteria. Charge transfer-polaron model.
Bioelectrochemistry 2003; 59: 113-9.
4. Novoderezhkin VI, Razjivin AP. Exciton dynamics
in circular aggregates: Application to antenna of photosynthetic purple
bacteria. Biophys J. 1995; 68: 1089-100.
5. Razjivin AP, Danielius
RV, Gadonas RA, Borisov
AYu,
6. Abdourakhmanov IA, Danielius RV, Razjivin
AP. Efficiency of excitation trapping by reaction centers of complex B890
from Chromatium minutissimum.
FEBS Lett. 1989; 245: 47-50.
7. Danielius RV, Mineyev
AP, Razjivin AP. The cooperativity phenomena in a pigment-protein complex
of light-harvesting antenna revealed by picosecond
absorbance difference spectroscopy FEBS Lett.
1989; 250: 183-6.
Primany recent publications:
1. Borisov AY, Sidorin
YM. The concept of energy migration and trapping in purple
bacteria. Charge transfer-polaron model.
Bioelectrochemistry 2003; 59: 113-9.
2. van Grondelle R, Novoderezhkin V. The Dynamics
of Excitation Energy Transfer in the LH1 and LH2 Light-Harvesting Complexes
of Photosynthetic Bacteria. Biochemistry 2001; 40: 15057-68.
3. Novoderezhkin V, van Grondelle R. Exciton-vibrational
relaxation and transient absorption dynamics in LH1 of Rhodopseudomonas viridis:
a Redfield theory approach. J Phys Chem. 2002: 106: 6025-37.
4. Novoderezhkin VI, Andrizhiyevskaya EG, Dekker
JP, van Grondelle R. Pathways and timescales
of primary charge separation in the photosystem
II reaction center as revealed by a simultaneous fit of time-resolved fluorescence
and transient absorption. Biophys J. 2005; 89(3):1464-81.
5. van Grondelle R, Novoderezhkin VI. Energy transfer in photosynthesis:
experimental insights and quantitative models. Phys Chem Chem Phys. 2006 Feb
21;8(7):793-807.
6. Mikhailyuk IK, Lokstein H, Razjivin
AP. A method of spectral subband
decomposition by simultaneous fitting the initial spectrum and a set of
its derivatives. J Biochem
Biophys Methods. 2005; 63(1):10-23.
7. Mikhailyuk IK,
Knox PP, Paschenko VZ, Razjivin
AP, Lokstein H. Analysis of absorption spectra
of purple bacterial reaction centers in the near infrared region by higher
order derivative spectroscopy. Biophys
Chem. 2006 Feb 27; [Epub ahead of print]
Some information about Department of photosynthesis
and activities of its reseachers may be find in: 1. Borisov AY. (2003) The
beginning of research on biophysics of photosynthesis and initial contributions
made by Russian scientists to its development. Photosynth
Res. 2003; 76: 413-26; 2. Razjivin A.P., Leupold D., and V.I. Novoderezhkin. 1998. Exciton
delocalization in antenna complexes. In: Photosynthesis: Mechanisms and
Effects. Garab G., editor. Kluwer Acad. Publ., Dorderecht, The