Документ взят из кэша поисковой машины. Адрес оригинального документа : http://mccmb.belozersky.msu.ru/2013/abstracts/abstracts/129.pdf Дата изменения: Mon Mar 25 18:15:56 2013 Дата индексирования: Thu Feb 27 20:56:28 2014 Кодировка:

Differential expression of glycolysis pathway genes in renal, lung and breast cancer . V. Kudryavtseva, . V. Snezhkina, . . Dmitriev, G.S. Krasnov, . F. Sadritdinova, N. V. Melnikova, A.O. Stepanov, L.A. Uroshlev, V.A. Lakunina, M.V. Darii., N. Yu. Oparina.
Engelhardt Institute of Molecular Biology RAS, Moscow, 119991 Russia, rhizamoeba@mail.ru

Glycolysis is one of the most highly conserved and ancient pathways. This is the major pathway of glucose catabolism accompanied by a synthesis of ATP. However, various tumors are characterized by frequently activated glycolysis even in aerobic conditions. Several glycolytic genes are highly expressed in all tissues and thus considered as housekeeping genes (such as GAPDH). At the same time, genes encoding other glycolytic enzymes are differentially expressed in a variety of normal and pathological cells. We have studied the transcriptomic data of human renal, lung and breast cancers and estimated the tissue and cancer specific glycolytic expression patterns. We have shown that in all studied neoplasms even the "core" glycolytic genes were differentially expressed in comparison to normal tissues. Among these genes we describe the overexpression of PKFP, ALDOA and GAPDH in renal cancer, significant upregulation of HK2 in breast cancer and GPI in squamous cell lung cancer. Several genes, such as ALDOA, ENO1, GAPDH, PGK1, were characterized by an unstable expression level with high variation in both normal and tumor samples. We have selected the subgroups of the probable housekeeping (reference) glycolytic genes for these cancers: HK1, ADPGK, GPI, PGK1, PKM2 for renal cancer; ADPGK, ALDOA, GAPDH, PGK1, BPGM, ENO1, PKM2 for squamous cell lung cancer and ADPGK, BPGM, ENO2, GPI, PKFM for breast cancer. The cases of decrease of glycolytic enzyme's mRNA level were rare. These data were validated using realtime PCR. Therefore, we have detected dysregulation of glycolysis in all studied cancers. This work was financially supported by a Grant of the President of Russian Federation 5666.2013.4 and RFFI 13-04-02072.