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Statistical analysis of nucleotide sequences of a cytochrome c oxidase subunit 1 (CO1) mtDNA arctic (Liopsetta glacialis) and striped (Liopsetta pinnifasciata) flounder (Pleuronectidae) of the Sea of Okhotsk S.P.Pustovoit
Institute of Biological Problems of the North FED, Russian Academy of Sciences, Portovaya Str. 18, Magadan 685000, Russia, e-mail:pustov@ibpn.ru

Within global project DNA- barcoding of animal species and plants (Hebert et al. 2003) in 2005 the program devoted to fishes (Fish Barcode of Life Initiative, FISH-BOL) (Ward et al. 2005). By July, 2011 from about 31 thousand types fishes the nucleotide sequence of a cytochrome c oxidase subunit 1 is known for 25% of species, and for order of the Pleuronectiformes - 30,2% (Becker et al., 2011). Individuals of striped (L.pinnifasciata) and arctic (L.glacialis) flounder are caught during the winter period from 2010-2012 in the Amakhtonsky gulf of the Taui lip, northern part of the Sea of Okhotsk. The purpose of article carry out the statistical analysis of new nucleotide sequences of a gene cytochrome c oxidase two species. Statistical analysis is carried out by the program MEGA 5.1. (Tamura et al., 2011). At 10 individuals of arctic flounder is revealed 6 gaplotyps (Potapova et al., 2013). Replacements of nucleotides are most adequately described by Tamura's model, T92 (T92, Tamura, 92). It is possible to judge suitability of this model in size of Bayesian criterion (BIC, Bayesian Information Criterion, BIC=1882,5). At 28 individuals of a striped flounder is revealed 11 gaplotypes. For a striped flounder the most probable way of replacement of nucleotides is the one-parametrical model Jukes and the Cantor (JC, BIC=2464,37). Table. Indicators of variability of nucleotide sequences of a gene cytochrome c oxidase 1 at arctic (L.glacialis) and striped (L.pinnifaciata) flounder N L.glacialis L.pinnifaciata 596 599 m 10 28 s 6 15 p
s

0 ,0 0 3 7 0 0 ,0 0 4 4 6

D 0 ,1 5 4 -1,046

model 92 JC(K2)

0 ,0 1 0 0 8 0 ,0 2 5 0 4


N- length of gene, m- number of fish (sequences), s- number of polymorphic sites, ps =s/N­ part of polymorphic sites, ­ nucleotide diversity, D- test Tajima, «model» - model of nucleotide substitutions. Nuleotide diversity of striped and arctic flounder differs slightly (table). Low level of differences of a nucleotide variety of two flounders is connected with that is the sibling species entering into one genus. Cytochrome c oxidase is considered a gene characteristic for each species (Shneyer, 2009), however results of our statistical analysis show that its variability isn't equal to zero. S.Becker et al. (2011).Five years of FISH-BOL: Brief status report, Mitochondrial DNA. 22(S1):3-9. P.D.N.Hebert et al. (2003). Biological identifications through DNA barcodes, Proceedings of the Royal Society of London, Series B. V.270. 1512: 313 - 321. V.S.Shneyer (2009). DNA barcoding of animal and plant species as an approach for their molecular identification and describing of diversity, Journal of General Biology,.70.4:296-315. K.Tamura et al. (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. www. megasoftware.net R.D.Ward et al. (2005). DNA barcoding Australia's fish species, Proceedings of the Royal Society of London. Series B. 360. 1716: P.1847 - 1857. N.A.Potapova et al. (2013). DNA barcoding of arctic flounder Liopsetta glacialis, Herald of SVGU. 19.: 22-30.