Документ взят из кэша поисковой машины. Адрес оригинального документа : http://mccmb.belozersky.msu.ru/2015/proceedings/abstracts/169.pdf Дата изменения: Mon Jun 15 15:40:13 2015 Дата индексирования: Sat Apr 9 23:37:39 2016 Кодировка:

Accumulation of mutations in nonsense alleles of Drosophila melanogaster Nadezhda A. Potapova
Northeastern State University, Magadan, Russia, nadezhdalpotapova@gmail.com

Maria A. Baranova
Department of Bioinformatics and Bioengineering, M. V. Lomonosov Moscow State University, Moscow, Russia, baranova.mariia@gmail.com

Georgii A. Bazykin
Department of Bioinformatics and Bioengineering, M. V. Lomonosov Moscow State University, Moscow, Russia; Sector for Molecular Evolution, Institute for Information Transmission Problems of the RAS (Kharkevich Institute), Moscow, Russia, gbazykin@iitp.ru

Alexey S. Kondrashov
Department of Bioinformatics and Bioengineering, M. V. Lomonosov Moscow Stat e University, Moscow, Russia; Department of Ecology and Evolutionary Biology and Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, United States of America,kondrash@umich.edu

Mutations are the sine qua non of evolution; they also shape variation. Even deleterious mutations may segregate within a population for multiple generations if selection against them is not too strong. Point mutations in coding regions of genes may be synonymous if they don't change the encoded amino acid; nonsynonymous if they change it; or nonsense if they result in a premature stop codon. As a nonsense mutation pseudogenizes the gene, it effectively disables negative selection at a gene, making subsequent accumulation of nonsynonymous mutations at other positions of the same gene neutral. Therefore, in the absence of recombination, post-nonsense nonsynonymous mutations are expected to accumulate at the same rate as synonymous mutations. Here, we verify this hypothesis using genomes of 162 inbred lines Drosophila melanogaster. We identified 960 genes with 1202 nonsense mutations. On average, each line carries 63 nonsense mutations in 59 genes. The number of nonsynonymous mutations nested within nonsense alleles may be used to estimate the age distribution of such mutations, and therefore, the period of time for which they segregate in the population.