Biological activity of novel synthetic derivatives of carnosine.

Stvolinsky SL; Bulygina ER; Fedorova TN; Meguro K; Sato T; Tyulina OV; Abe H; Boldyrev AA

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Article

Title:	Biological activity of novel synthetic derivatives of carnosine.
Authors:	Stvolinsky SL; Bulygina ER; Fedorova TN; Meguro K; Sato T; Tyulina OV; Abe H; Boldyrev AA
Publication:	Cell Mol Neurobiol. 2010 Apr;30(3):395-404. doi: 10.1007/s10571-009-9462-7. Epub 2009 Oct 2.
PubmedID	19798566
Abstract
Two novel derivatives of carnosine--(S)-trolox-L-carnosine (STC) and (R)-trolox-L-carnosine (RTC) are characterized in terms of their antioxidant and membrane-stabilizing activities as well as their resistance to serum carnosinase. STC and RTC were synthesized by N-acylation of L-carnosine with (S)- and (R)-trolox, respectively. STC and RTC were found to react more efficiently with 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and protect serum lipoproteins from Fe(2+)-induced oxidation more successfully than carnosine and trolox. At the same time, STC, RTC and trolox suppressed oxidative hemolysis of red blood cells (RBC) less efficiently than carnosine taken in the same concentration. When oxidative stress was induced in suspension of cerebellum granule cells by their incubation with N-methyl-D-aspartate (NMDA), or hydrogen peroxide (H(2)O(2)), both STC and RTC more efficiently decreased accumulation of reactive oxygen species (ROS) than carnosine and trolox. Both STC and RTC were resistant toward hydrolytic degradation by human serum carnosinase. STC and RTC were concluded to demonstrate higher antioxidant capacity and better ability to prevent cerebellar neurons from ROS accumulation than their precursors, carnosine and trolox.