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TmrnaEnglish 9 - 2009-10-15 - Main.AndreyGolovin

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tmRNA

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To visualize tmRNA on the ribosome at the elongation steps of trans-translation we have applied cryo-electron tomography. It should be mentioned that we made many attempts to use single particle reconstitution cryo-EM to study these complexes but were unable to detect clear tmRNA density suggesting tmRNA arch flexibility. Although cryo-EM tomography does not allow getting a high resolution structure it allows to visualize a single particle (Zhao et al. 2004a; Zhao et al. 2004b) and to follow particular tmRNA conformations in different ribosomal particles. Indeed we have found that the tmRNA arch can move from the shoulder to the top of the head of the 30S ribosomal subunit (Fig. 1).

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Figure 1. Cry-electron tomography of tmRNA on the ribosome.

Revision 9	r9 - 2009-10-15 - 20:48:48 - AndreyGolovin
Revision 8	r8 - 2009-10-15 - 09:34:37 - OlgaShpanchenko

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