ÈÑÒÈÍÀ

The present invention provides constructs and methods for expression and accumulation of tuberculosis vaccine proteins in tissue or organ of a plant. The proteins are obtained by transient expression in and/or stable transformation of plants with non-replicating genes or virus-based vector cDNAs encoding the proteins. The genes include cellular compartment-specific, regulatory and/or affinity purification tag sequences. The plant tissue fractions containing the tuberculosis vaccine proteins themselves or after purification are useful as a tuberculosis vaccine.