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ID HSLV_ECOLI Reviewed; 176 AA.
AC P0A7B8; P31059; P97542; Q2M8M8;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 22-SEP-2009, entry version 49.
DE RecName: Full=ATP-dependent protease hslV;
DE EC=3.4.25.-;
DE AltName: Full=Heat shock protein hslV;
GN Name=hslV; Synonyms=htpO, yiiC; OrderedLocusNames=b3932, JW3903;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX MEDLINE=94063501; PubMed=8244018; DOI=10.1016/0378-1119(93)90167-2;
RA Chuang S.E., Burland V., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT "Sequence analysis of four new heat-shock genes constituting the
RT hslTS/ibpAB and hslVU operons in Escherichia coli.";
RL Gene 134:1-6(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX MEDLINE=93347969; PubMed=8346018; DOI=10.1093/nar/21.15.3391;
RA Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome. III. DNA sequence of the
RT region from 87.2 to 89.2 minutes.";
RL Nucleic Acids Res. 21:3391-3398(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
RT MG1655 and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125.
RX MEDLINE=97166005; PubMed=9013898; DOI=10.1016/S0014-5793(96)01485-8;
RA Ohya S., Takii T., Yamazaki H., Matsumori M., Onozaki K., Watanabe M.,
RA Imaizumi Y.;
RT "Molecular cloning of a novel gene involved in serotonin receptor-
RT mediated signal transduction in rat stomach.";
RL FEBS Lett. 401:252-258(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-88.
RA Dai K., Xu Y., Lutkenhaus J.;
RL Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP MUTAGENESIS OF THR-2; THR-3; SER-6; SER-104; SER-125; SER-144 AND
RP SER-173.
RX MEDLINE=97400195; PubMed=9257689; DOI=10.1016/S0014-5793(97)00742-4;
RA Yoo S.J., Shim Y.K., Seong I.S., Seol J.H., Kang M.S., Chung C.H.;
RT "Mutagenesis of two N-terminal Thr and five Ser residues in HslV, the
RT proteolytic component of the ATP-dependent HslVU protease.";
RL FEBS Lett. 412:57-60(1997).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS).
RX MEDLINE=97322325; PubMed=9177170; DOI=10.1073/pnas.94.12.6070;
RA Bochtler M., Ditzel L., Groll M., Huber R.;
RT "Crystal structure of heat shock locus V (HslV) from Escherichia
RT coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:6070-6074(1997).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.81 ANGSTROMS) OF COMPLEX WITH HSLU.
RX MEDLINE=20155479; PubMed=10693812; DOI=10.1038/35001629;
RA Bochtler M., Hartmann C., Song H.K., Bourenkov G.P., Bartunik H.D.,
RA Huber R.;
RT "The structures of HslU and the ATP-dependent protease HslU-HslV.";
RL Nature 403:800-805(2000).
CC -!- FUNCTION: Protease subunit of a proteasome-like degradation
CC complex.
CC -!- SUBUNIT: A double ring-shaped homohexamer of hslV is capped on
CC each side by a ring-shaped hslU homohexamer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the peptidase T1B family. HslV subfamily.
CC -!- CAUTION: PubMed:9013898 sequence is supposed to originate from rat
CC but, based on sequence similarity, it seems that this is a case of
CC bacterial contamination from E.coli.
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DR EMBL; L19201; AAB03064.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76914.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77378.1; -; Genomic_DNA.
DR EMBL; D89965; BAA14040.1; ALT_SEQ; mRNA.
DR EMBL; L14281; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; JT0760; JT0760.
DR RefSeq; AP_003877.1; -.
DR RefSeq; NP_418367.1; -.
DR PDB; 1E94; X-ray; 2.80 A; A/B/C/D=2-176.
DR PDB; 1G4A; X-ray; 3.00 A; A/B/C/D=2-175.
DR PDB; 1G4B; X-ray; 7.00 A; M/N/O/P=2-175.
DR PDB; 1HQY; X-ray; 2.80 A; A/B/C/D=2-176.
DR PDB; 1HT1; X-ray; 2.80 A; A/B/C/D/V/X/Y/Z=2-176.
DR PDB; 1HT2; X-ray; 2.80 A; A/B/C/D/I/J/K/L=2-176.
DR PDB; 1NED; X-ray; 3.80 A; A/B/C=2-176.
DR PDBsum; 1E94; -.
DR PDBsum; 1G4A; -.
DR PDBsum; 1G4B; -.
DR PDBsum; 1HQY; -.
DR PDBsum; 1HT1; -.
DR PDBsum; 1HT2; -.
DR PDBsum; 1NED; -.
DR STRING; P0A7B8; -.
DR SWISS-2DPAGE; P0A7B8; -.
DR ECO2DBASE; G021.0; 6TH EDITION.
DR PRIDE; P0A7B8; -.
DR GeneID; 948429; -.
DR GenomeReviews; AP009048_GR; JW3903.
DR GenomeReviews; U00096_GR; b3932.
DR KEGG; ecj:JW3903; -.
DR KEGG; eco:b3932; -.
DR EchoBASE; EB1627; -.
DR EcoGene; EG11676; hslV.
DR HOGENOM; P0A7B8; -.
DR OMA; P0A7B8; AADICVY.
DR BioCyc; EcoCyc:EG11676-MON; -.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0005839; C:proteasome core complex; IEA:InterPro.
DR GO; GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0051603; P:proteolysis involved in cellular protein ca...; IEA:InterPro.
DR GO; GO:0006950; P:response to stress; IEA:UniProtKB-KW.
DR HAMAP; MF_00248; -; 1.
DR InterPro; IPR001353; Proteasome_sua/b.
DR Pfam; PF00227; Proteasome; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Protease;
KW Stress response; Threonine protease.
FT INIT_MET 1 1 Removed (Probable).
FT CHAIN 2 176 ATP-dependent protease hslV.
FT /FTId=PRO_0000148105.
FT ACT_SITE 2 2
FT STRAND 4 7
FT STRAND 12 17
FT STRAND 23 25
FT TURN 39 42
FT TURN 51 54
FT HELIX 55 68
FT HELIX 74 77
FT HELIX 80 83
FT STRAND 86 88
FT STRAND 106 108
FT TURN 109 111
FT STRAND 112 114
FT TURN 117 119
FT HELIX 127 138
FT HELIX 145 159
FT STRAND 168 173
SQ SEQUENCE 176 AA; 19093 MW; 3B35E01F51486965 CRC64;
MTTIVSVRRN GHVVIAGDGQ ATLGNTVMKG NVKKVRRLYN DKVIAGFAGG TADAFTLFEL
FERKLEMHQG HLVKAAVELA KDWRTDRMLR KLEALLAVAD ETASLIITGN GDVVQPENDL
IAIGSGGPYA QAAARALLEN TELSAREIAE KALDIAGDIC IYTNHFHTIE ELSYKA
//