Документ взят из кэша поисковой машины. Адрес оригинального документа : http://kodomo.cmm.msu.ru/~sds/Supplement_materials/term3/block1/21.txt Дата изменения: Fri Oct 6 19:16:16 2006 Дата индексирования: Wed Jan 14 09:31:21 2009 Кодировка:

BLASTP 2.2.10 [Oct-19-2004]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= KPN2Jun2003_21 [4410 - 2881] (REVERSE SENSE)
(510 letters)

Database: ep
4855 sequences; 1,534,022 total letters

Searching..........done

Score E
Sequences producing significant alignments: (bits) Value

PTGCB_ECOLI P69786 PTS system glucose-specific EIICB component (... 372 e-104
PTW3C_ECOLI P09323 PTS system N-acetylglucosamine-specific EIICB... 314 9e-87
PTOCB_ECOLI P19642 PTS system maltose- and glucose-specific EIIC... 304 1e-83


>PTGCB_ECOLI P69786 PTS system glucose-specific EIICB component
(EIICB-Glc) (EII-Glc) [Includes: Glucose permease IIC
component (PTS system glucose-specific EIIC component);
Glucose-specific phosphotransferase enzyme IIB component
(EC 2.7.1.69) (PTS system glucose-specific EIIB
component)].
Length = 477

Score = 372 bits (956), Expect = e-104
Identities = 207/492 (42%), Positives = 303/492 (61%), Gaps = 30/492 (6%)

Query: 7 NLLQMLQSIGRSLMIPIAMLPAAGILLAFGVSFQDPNIVASLPFLGADWLVHVLK-LMAE 65
N LQ +G+SLM+P+++LP AGILL G + WL V+ +MAE
Sbjct: 4 NAFANLQKVGKSLMLPVSVLPIAGILLGVGSA-------------NFSWLPAVVSHVMAE 50

Query: 66 AGSVIFANLPLLFAVGVAVGLSDDQGIAGLSAIAGFLIMNVTIGQFLGITPESVAQVRDY 125
AG +FAN+PL+FA+GVA+G +++ G++ L+A+ + IM T+ + A+
Sbjct: 51 AGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEE--- 107

Query: 126 TMVLGIPSLQTGVFXXXXXXXXAAWLYKRYYRIQLPSWLEFFSGKRFVPIITSFAALFVG 185
+ TGV AA+++ R+YRI+LP +L FF+GKRFVPII+ AA+F G
Sbjct: 108 --IASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTG 165

Query: 186 LVMAVVWPPVQHLINGLSNTMTVQGPGVSAFLFGFVEQLLIPFGLNHVWWPTFWLQFGEY 245
+V++ +WPP+ I S Q P V+ ++GF+E+ L+PFGL+H+W F +Q GEY
Sbjct: 166 VVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEY 225

Query: 246 VNKAGQVVHGDQLIFFAQLKDQVPITAGTFMAGLTPIKMFCIPAIALAIYRCASPENRAR 305
N AGQV HGD + A TAG G KM+ +PA A+AI+ A PENRA+
Sbjct: 226 TNAAGQVFHGDIPRYMAG-----DPTAGKLSGGFL-FKMYGLPAAAIAIWHSAKPENRAK 279

Query: 306 VKGIMLSGAITSIVCGITEPIEFSFLFVAPVLYGIHAVLAGLVFLLMEWFSVHIGLSFSG 365
V GIM+S A+TS + GITEPIEFSF+FVAP+LY IHA+LAGL F + + G SFS
Sbjct: 280 VGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSH 339

Query: 366 GLIDYLFFGVLPRAPHWYMVFP-VGLVMGALYYALFTFAIRSWNLLTPGREVEENAVAQE 424
GLID++ VL +FP VG+ +YY +F I++ +L TPGRE + A+
Sbjct: 340 GLIDFI---VLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGRE-DATEDAKA 395

Query: 425 SEQNDLVSGIILAYGGLGNMTSIEACMSRLRIDVTDKTLVDKALLKQLGAAGIVEVGNNI 484
+ +++ ++ A+GG N+T+++AC++RLR+ V D + VD+A LK+LGAAG+V G+ +
Sbjct: 396 TGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGV 455

Query: 485 QSVFGMKSDRLK 496
Q++FG KSD LK
Sbjct: 456 QAIFGTKSDNLK 467



Database: ep
Posted date: Oct 3, 2006 10:20 PM
Number of letters in database: 1,534,022
Number of sequences in database: 4855

Lambda K H
0.327 0.142 0.434

Gapped
Lambda K H
0.267 0.0410 0.140


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,540,376
Number of Sequences: 4855
Number of extensions: 68823
Number of successful extensions: 276
Number of sequences better than 1.0e-03: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 244
Number of HSP's gapped (non-prelim): 9
length of query: 510
length of database: 1,534,022
effective HSP length: 89
effective length of query: 421
effective length of database: 1,101,927
effective search space: 463911267
effective search space used: 463911267
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 89 (38.9 bits)