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ID PTHP_ECOLI Reviewed; 85 AA.
AC P0AA04; P05525; P07006;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 22-SEP-2009, entry version 43.
DE RecName: Full=Phosphocarrier protein HPr;
DE EC=2.7.11.-;
DE AltName: Full=Histidine-containing protein;
GN Name=ptsH; Synonyms=hpr; OrderedLocusNames=b2415, JW2408;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=88314869; PubMed=2457575;
RA de Reuse H., Danchin A.;
RT "The ptsH, ptsI, and crr genes of the Escherichia coli
RT phosphoenolpyruvate-dependent phosphotransferase system: a complex
RT operon with several modes of transcription.";
RL J. Bacteriol. 170:3827-3837(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=85286351; PubMed=2411636; DOI=10.1016/0378-1119(85)90172-6;
RA de Reuse H., Roy A., Danchin A.;
RT "Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12:
RT nucleotide sequence of the ptsH gene.";
RL Gene 35:199-207(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=88058992; PubMed=2960675;
RA Saffen D.W., Presper K.A., Doering T.L., Roseman S.;
RT "Sugar transport by the bacterial phosphotransferase system. Molecular
RT cloning and structural analysis of the Escherichia coli ptsH, ptsI,
RT and crr genes.";
RL J. Biol. Chem. 262:16241-16253(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX MEDLINE=88257033; PubMed=3290198;
RA Byrne C.R., Monroe R.S., Ward K.A., Kredich N.M.;
RT "DNA sequences of the cysK regions of Salmonella typhimurium and
RT Escherichia coli and linkage of the cysK regions to ptsH.";
RL J. Bacteriol. 170:3150-3157(1988).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX MEDLINE=97349980; PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K.,
RA Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N.,
RA Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H.,
RA Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S.,
RA Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C.,
RA Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-
RT K12 genome corresponding to 50.0-68.8 min on the linkage map and
RT analysis of its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA Mau B., Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1474(1997).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains
RT MG1655 and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [8]
RP PROTEIN SEQUENCE OF 1-20.
RC STRAIN=K12;
RX MEDLINE=99085675; PubMed=9868784;
RX DOI=10.1111/j.1574-6968.1998.tb13343.x;
RA Wasinger V.C., Humphery-Smith I.;
RT "Small genes/gene-products in Escherichia coli K-12.";
RL FEMS Microbiol. Lett. 169:375-382(1998).
RN [9]
RP PROTEIN SEQUENCE OF 1-12.
RC STRAIN=K12 / EMG2;
RX MEDLINE=97443975; PubMed=9298646; DOI=10.1002/elps.1150180807;
RA Link A.J., Robison K., Church G.M.;
RT "Comparing the predicted and observed properties of proteins encoded
RT in the genome of Escherichia coli K-12.";
RL Electrophoresis 18:1259-1313(1997).
RN [10]
RP PROTEIN SEQUENCE OF 1-12.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F.;
RL Submitted (FEB-1996) to UniProtKB.
RN [11]
RP ACTIVE SITE HIS-15.
RX MEDLINE=91084491; PubMed=2261470; DOI=10.1021/bi00487a026;
RA van Dijk A.A., de Lange L.C., Bachovchin W.W., Robillard G.T.;
RT "Effect of phosphorylation on hydrogen-bonding interactions of the
RT active site histidine of the phosphocarrier protein HPr of the
RT phosphoenolpyruvate-dependent phosphotransferase system determined by
RT 15N NMR spectroscopy.";
RL Biochemistry 29:8164-8171(1990).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RX MEDLINE=94043003; PubMed=8226757;
RA Jia Z., Quail J.W., Waygood E.B., Delbaere L.T.J.;
RT "The 2.0-A resolution structure of Escherichia coli histidine-
RT containing phosphocarrier protein HPr. A redetermination.";
RL J. Biol. Chem. 268:22490-22501(1993).
RN [13]
RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
RX MEDLINE=96378616; PubMed=8784179; DOI=10.1021/bi9603480;
RA Napper S., Anderson J.W., Georges F., Quail J.W., Delbaere L.T.J.,
RA Waygood E.B.;
RT "Mutation of serine-46 to aspartate in the histidine-containing
RT protein of Escherichia coli mimics the inactivation by phosphorylation
RT of serine-46 in HPrs from Gram-positive bacteria.";
RL Biochemistry 35:11260-11267(1996).
RN [14]
RP STRUCTURE BY NMR.
RX MEDLINE=87101066; PubMed=3542036; DOI=10.1021/bi00371a073;
RA Klevit R.E., Waygood E.B.;
RT "Two-dimensional 1H NMR studies of histidine-containing protein from
RT Escherichia coli. 3. Secondary and tertiary structure as determined by
RT NMR.";
RL Biochemistry 25:7774-7781(1986).
RN [15]
RP STRUCTURE BY NMR.
RX MEDLINE=92089095; PubMed=1751501; DOI=10.1021/bi00115a014;
RA Hammen P.K., Waygood E.B., Klevit R.E.;
RT "Reexamination of the secondary and tertiary structure of histidine-
RT containing protein from Escherichia coli by homonuclear and
RT heteronuclear NMR spectroscopy.";
RL Biochemistry 30:11842-11850(1991).
RN [16]
RP STRUCTURE BY NMR.
RX MEDLINE=93130914; PubMed=1483471;
RX DOI=10.1111/j.1432-1033.1992.tb17492.x;
RA van Nuland N.A.J., Groetzinger J., Dijkstra K., Scheek R.M.,
RA Robillard G.T.;
RT "Determination of the three-dimensional solution structure of the
RT histidine-containing phosphocarrier protein HPr from Escherichia coli
RT using multidimensional NMR spectroscopy.";
RL Eur. J. Biochem. 210:881-891(1992).
RN [17]
RP STRUCTURE BY NMR.
RX MEDLINE=94210480; PubMed=8158637; DOI=10.1006/jmbi.1994.1254;
RA van Nuland N.A.J., Hangyi I.W., van Schaik R.C., Berendsen H.J.,
RA van Gunsteren W.F., Scheek R.M., Robillard G.T.;
RT "The high-resolution structure of the histidine-containing
RT phosphocarrier protein HPr from Escherichia coli determined by
RT restrained molecular dynamics from nuclear magnetic resonance nuclear
RT Overhauser effect data.";
RL J. Mol. Biol. 237:544-559(1994).
RN [18]
RP STRUCTURE BY NMR.
RX MEDLINE=95156481; PubMed=7853396; DOI=10.1006/jmbi.1994.0075;
RA van Nuland N.A.J., Boelens R., Scheek R.M., Robillard G.T.;
RT "High-resolution structure of the phosphorylated form of the
RT histidine-containing phosphocarrier protein HPr from Escherichia coli
RT determined by restrained molecular dynamics from NMR-NOE data.";
RL J. Mol. Biol. 246:180-193(1995).
RN [19]
RP STRUCTURE BY NMR.
RX MEDLINE=97022120; PubMed=8868480;
RA van Nuland N.A.J., Wiersma J.A., van der Spoel D., de Groot B.L.,
RA Scheek R.M., Robillard G.T.;
RT "Phosphorylation-induced torsion-angle strain in the active center of
RT HPr, detected by NMR and restrained molecular dynamics refinement.";
RL Protein Sci. 5:442-446(1996).
RN [20]
RP STRUCTURE BY NMR.
RX MEDLINE=99140298; PubMed=10048929; DOI=10.1038/5854;
RA Garrett D.S., Seok Y.-J., Peterkofsky A., Gronenborn A.M., Clore G.M.;
RT "Solution structure of the 40,000 Mr phosphoryl transfer complex
RT between the N-terminal domain of enzyme I and HPr.";
RL Nat. Struct. Biol. 6:166-173(1999).
CC -!- FUNCTION: General (non sugar-specific) component of the
CC phosphoenolpyruvate-dependent sugar phosphotransferase system
CC (sugar PTS). This major carbohydrate active-transport system
CC catalyzes the phosphorylation of incoming sugar substrates
CC concomitantly with their translocation across the cell membrane.
CC The phosphoryl group from phosphoenolpyruvate (PEP) is transferred
CC to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr
CC then transfers it to the permease (enzymes II/III).
CC -!- CATALYTIC ACTIVITY: Protein HPr N(pi)-phospho-L-histidine +
CC protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-
CC histidine.
CC -!- INTERACTION:
CC P69783:crr; NbExp=1; IntAct=EBI-902853, EBI-369856;
CC P08839:ptsI; NbExp=1; IntAct=EBI-902853, EBI-551533;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the HPr family.
CC -!- SIMILARITY: Contains 1 HPr domain.
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DR EMBL; M10425; AAA24438.1; -; Genomic_DNA.
DR EMBL; J02796; AAA24440.1; -; Genomic_DNA.
DR EMBL; M21994; AAA24384.1; -; Genomic_DNA.
DR EMBL; M21451; AAA23655.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75468.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16289.1; -; Genomic_DNA.
DR PIR; A29785; WQECPH.
DR RefSeq; AP_003009.1; -.
DR RefSeq; NP_416910.1; -.
DR PDB; 1CM2; X-ray; 1.80 A; A=1-85.
DR PDB; 1CM3; X-ray; 1.60 A; A=1-85.
DR PDB; 1GGR; NMR; -; B=1-85.
DR PDB; 1HDN; NMR; -; A=1-85.
DR PDB; 1J6T; NMR; -; B=1-85.
DR PDB; 1OPD; X-ray; 1.50 A; A=1-85.
DR PDB; 1PFH; NMR; -; A=1-85.
DR PDB; 1POH; X-ray; 2.00 A; A=1-85.
DR PDB; 1VRC; NMR; -; C/D=1-85.
DR PDB; 2JEL; X-ray; 2.50 A; P=1-85.
DR PDB; 3CCD; X-ray; 1.00 A; A/B=1-85.
DR PDB; 3EZA; NMR; -; B=1-85.
DR PDB; 3EZB; NMR; -; B=1-85.
DR PDB; 3EZE; NMR; -; B=1-85.
DR PDBsum; 1CM2; -.
DR PDBsum; 1CM3; -.
DR PDBsum; 1GGR; -.
DR PDBsum; 1HDN; -.
DR PDBsum; 1J6T; -.
DR PDBsum; 1OPD; -.
DR PDBsum; 1PFH; -.
DR PDBsum; 1POH; -.
DR PDBsum; 1VRC; -.
DR PDBsum; 2JEL; -.
DR PDBsum; 3CCD; -.
DR PDBsum; 3EZA; -.
DR PDBsum; 3EZB; -.
DR PDBsum; 3EZE; -.
DR IntAct; P0AA04; 2.
DR STRING; P0AA04; -.
DR TCDB; 8.A.8.1.1; phosphotransferase system HPr family.
DR PhosSite; P0AA04; -.
DR SWISS-2DPAGE; P0AA04; -.
DR ECO2DBASE; F007.0; 6TH EDITION.
DR PRIDE; P0AA04; -.
DR GeneID; 946886; -.
DR GenomeReviews; AP009048_GR; JW2408.
DR GenomeReviews; U00096_GR; b2415.
DR KEGG; ecj:JW2408; -.
DR KEGG; eco:b2415; -.
DR EchoBASE; EB0781; -.
DR EcoGene; EG10788; ptsH.
DR HOGENOM; P0AA04; -.
DR OMA; P0AA04; QGTILTI.
DR BioCyc; EcoCyc:PTSH-MON; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0005515; F:protein binding; IPI:IntAct.
DR GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phospho...; IEA:UniProtKB-KW.
DR InterPro; IPR001020; PTS_HPr_His_P_site.
DR InterPro; IPR005698; PTS_HPr_prot.
DR InterPro; IPR000032; PTS_HPr_prot-like.
DR InterPro; IPR002114; PTS_HPr_Ser_P_site.
DR Gene3D; G3DSA:3.30.1340.10; PTS_HPr_protein; 1.
DR Pfam; PF00381; PTS-HPr; 1.
DR PRINTS; PR00107; PHOSPHOCPHPR.
DR ProDom; PD002238; HPr_protein; 1.
DR TIGRFAMs; TIGR01003; PTS_HPr_family; 1.
DR PROSITE; PS51350; PTS_HPR_DOM; 1.
DR PROSITE; PS00369; PTS_HPR_HIS; 1.
DR PROSITE; PS00589; PTS_HPR_SER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing;
KW Kinase; Phosphotransferase system; Sugar transport; Transferase;
KW Transport.
FT CHAIN 1 85 Phosphocarrier protein HPr.
FT /FTId=PRO_0000107850.
FT DOMAIN 1 85 HPr.
FT ACT_SITE 15 15 Pros-phosphohistidine intermediate.
FT STRAND 2 7
FT HELIX 16 26
FT STRAND 31 37
FT STRAND 40 43
FT HELIX 47 50
FT STRAND 60 67
FT HELIX 70 83
SQ SEQUENCE 85 AA; 9119 MW; E5C8879C0A95AD41 CRC64;
MFQQEVTITA PNGLHTRPAA QFVKEAKGFT SEITVTSNGK SASAKSLFKL QTLGLTQGTV
VTISAEGEDE QKAVEHLVKL MAELE
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