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: http://www.biochem.bio.msu.ru/publications/publication.php?pubmedID=18037377
Дата изменения: Unknown Дата индексирования: Mon Oct 1 20:41:22 2012 Кодировка: |
Title: | Reversible dissociation of flavin mononucleotide from the mammalian membrane-bound NADH: ubiquinone oxidoreductase (complex I). |
Authors: | Gostimskaya IS; Grivennikova VG; Cecchini G; Vinogradov AD |
Publication: | FEBS Lett. 2007 Dec 22;581(30):5803-6. Epub 2007 Nov 26. |
PubmedID | 18037377 |
Abstract | |
Conditions for the reversible dissociation of flavin mononucleotide (FMN) from the membrane-bound mitochondrial NADH:ubiquinone oxidoreductase (complex I) are described. The catalytic activities of the enzyme, i.e. rotenone-insensitive NADH:hexaammineruthenium III reductase and rotenone-sensitive NADH:quinone reductase decline when bovine heart submitochondrial particles are incubated with NADH in the presence of rotenone or cyanide at alkaline pH. FMN protects and fully restores the NADH-induced inactivation whereas riboflavin and flavin adenine dinucleotide do not. The data show that the reduction of complex I significantly weakens the binding of FMN to protein thus resulting in its dissociation when the concentration of holoenzyme is comparable with K(d ( approximately 10(-8)M at pH 10.0). |