Measurement of B-type natriuretic peptide by two assays utilizing antibodies with different epitope specificity.

�� . �� : http://www.biochem.bio.msu.ru/publications/publication.php?pubmedID=20950595
�� : Unknown
�� : Sun Feb 3 00:13:20 2013
��:

Article

Title:	Measurement of B-type natriuretic peptide by two assays utilizing antibodies with different epitope specificity.
Authors:	Tamm NN; Semenov AG; Seferian KR; Bereznikova AV; Murakami MM; Apple FS; Koshkina EV; Krasnoselsky MI; Katrukha AG
Publication:	Clin Biochem. 2011 Feb;44(2-3):257-9. doi: 10.1016/j.clinbiochem.2010.09.030. Epub 2010 Oct 13.
PubmedID	20950595
Abstract
OBJECTIVES: To compare plasma BNP values determined by a conventional-type and a novel-type of BNP assays. DESIGN AND METHODS: Plasma samples (n=94) from HF patients were analyzed by the novel-type "Single Epitope Sandwich" (SES assay prototype) and the conventional-type Siemens ADVIA Centaur BNP assays. Both assays were calibrated using recombinant proBNP (expressed in E. coli). RESULTS: The SES assay measured 1.2- to 7.2-fold (2.1+/-0.9; mean, SD) greater BNP concentrations compared to the Siemens assay. A subset of six samples (6.4%) demonstrated the largest (3- to 7.2-fold higher) difference. CONCLUSIONS: The SES prototype assay appears to more accurately measure the absolute concentrations of BNP immunoreactive forms.