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Moscow University Chemistry Bulletin Vol. 51, No. 3, P. 235 (2010)
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M. M. Ulyashova, Yu. Yu. Ryabova, M. Yu. Rubtsova, A. M. Egorov

DNA-microarrays on porous membrane supports with colorimetric detection

Abstract

The technology of DNA-microarrays on porous membrane supports with colorimetric detection based on horseradish peroxidase was developed. The comparison of the methods of oligonucleotide immobilization on chemically different membranes was carried out, the conditions of colorimetric detection of biotin in DNA duplexes on microarray surface were optimized. The method developed was applied for determining type of genes encoding extended-spectrum b-lactamases.
Moscow University Chemistry Bulletin.
2010, Vol. 51, No. 3, P. 235
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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