Документ взят из кэша поисковой машины. Адрес оригинального документа : http://www.enzyme.chem.msu.ru/eduproc/Fluorescence.pdf Дата изменения: Tue May 13 20:58:56 2003 Дата индексирования: Mon Oct 1 20:48:42 2012 Кодировка:











dI A = Ikc' dl IT =e IO
- kc 'l '

k = 2300 / N I0 lg = lc I


0 = (1 + K D [Q ])(1 + K S [Q 0 = 1 + (K D + K
S

]) ]2
=

)[Q ]
app

+ K D K S [Q

= 1+ K K
app

[Q]
S

0 1 = - 1 [Q ] = (K D + K

)

+ K D K S [Q

]








COOH
COOH NH

COOH

NH
HO NH
2

2

2

N H


H

H

O C NH N

H2N

N N N

NH
2

2

N O OH P O O OH P O OH O CH N
2

O H3C N N N O H2C O P

N

O

OH

N

H2C OH O OH

N
H3C N

O
HO O OH OH OH

HO O P

OH

O

OH

O



1
-O

H2N O P O ON H+ CH3 H2 C CH2 OH

2
-O

O O P O ON H+ H2 C

H

OH

CH3

3 , b


tRNA






O

O

OH

COOH

N

C

S

(H3C)2N+

O

N(CH3)2

COOH

S

C

N

H3C

N

CH

3

SO3Cl
























R0 = 8.79 в 10
J=

-5

(Jq

D

n -4 k

2 1/ 6

)



A ( ) f D ( )4 d

f ( )d
D

k 2 = (cos

DA

- 3 cos D cos

A

)2

I Da E = 1 - ID

Da = 1- D


HIGH THROUGHPUT SCREENING
POPULATION SCREENING DRUG DESIGN









CATEGORIZED MICROPARTICLES Categories 1 2 3 4 5 6 Dansyl low high low Fluorescein low low high






. «», , 1972 . . .. .. « », 1980 . «», , 1979 J.Lakowicz Principles of fluorescent spectroscopy Second ed. Kluwer Academic/Plenum Publishers, New-York, 1999 R.Hagland Handbook of fluorescent probes and research chemicals. Molecular Probes, Inc. http://www.probes.com M.Zimmer Green Fluorescent Protein (GFP): Applications, Structure and Related Photophysical Behavior Chemical Review, 2002 P.Selvin The renaissance of fluorescence resonance energy transfer. Nature structural biology. V.7, number 9, pp.73-04, 2000 R.Rigler Fluorescence correlation, single molecule detection and large number screening. Applications in biotechnology J.Biotechnol., V.41, pp. 177-186, 1995 Evident Nanocrystals Catalog http://www.evidenttech.com