Prof. Lariononva N.I.

Prof. Lariononva N.I.

Head of the research group, leading scientist

e-mail: nilar(at)enzyme.chem.msu.ru

    Larionova N.I., Doctor of Philosophy since 1971, Doctor of Science since 1991, Professor since 2001, has over 200 scientific publications in Russian and International Journals. Larionova N.I. actively cooperates with foreign colleagues and has joint publications with scientists from the USA, France, Germany & Cuba. Hirsch index that quantifies both the actual scientific productivity and the apparent scientific impact of a scientist is 12. Larionova N.I. has founded a novel trend at Chemistry department in immobilized proteins usage in developing prolonged & targeted protein delivery systems.
    The scientific group headed by Larionova N.I. has completed a number of major studies in kinetics & mechanism of highly specific protein-protein interactions; structure and functioning of cathepsin G-like proteinases; molecular design of protein inhibitor conjugates; encapsulation of proteins, enzymes & poorly soluble anticancer agents; construction of structural elements for the endo-cardial electrode pacemakers containing dexamethasone. Scientific developments of Larionova N.I. are protected by 10 USSR Author's Certificates, 4 Russian, 2 foreign & 1 international PCT Patents.
    For the last 10 years 17 grants including RFBS, NATO, The Ministry of Education and Science of the Russian Federation were awarded the research group.
    Larionova N.I. is a member of Controlled Release Society, Bioencapsulation Research Group, the Board of Trustees & the Council of Experts of I.V.Berezin Foundation.
    Larionova N.I. is a reviewer for Boichemistry Moscow, Biochimia et Biophysica Acta, J. Nanosci. Nanotech., Current Medicinal Chemistry, J. Pharmacy and Pharmacology, member of the Editorial Boards of J. Drug Delivery Science and Technology, Acta Pharmaceutica Sciencia.
In 1999 Larionova N.I. was the Co-chairman of the Organizing Committee of an international symposium "Lipid and Surfactant Dispersed Systems".

    N.I.Larionova pays much attention to work with undergraduate & postgraduate students. 4 students and 2 PhD-students were awarded I.V.Berezin prize for their works carried out under her supervision. Larionova N.I. is the author of 6 manuals.

    29 students and 9 PhD-students have completed their degrees under the supervision of N.I.Larionova (see section "Graduates").

    Selected articles:

1. Balabushevich N.G., Larionоva N.I. Protein-loaded microspheres prepared by sequential adsorption of dextran sulphate and protamine on melamine formaldehyde core. Journal of Microencapsulation. DOI: 10.1080/02652040802518145 (в печати).
2. Larionova N.I., Zubaerova D.K., Guranda D.T.,. Pechyonkin M.A., Balabushevich N.G.
Colorimetric assay of chitosan in presence of proteins and polyelectrolytes by using o-phthalaldehyde.
Carbohyd. Polym., 2009, v. 75, N 4, p 724-727.
3. Zubaerova D.Kh., Larionova N.I.
Noninvasive Insulin Delivery Systems.
Biomed Khim. 2008, v. 54, N 3, 346-355.
4. Kuskov A.N., Villemson A.L., Shtilman M.I., Larionova N.I., Tsatsakis A.M., Tsikalas I., Rizos A.K.
Amphiphilic poly-N-vinylpyrrolidone nanocarriers with incorporated model proteins.
J. Phys-Condens. Mat., 2007, v. 19, N 20, Article number 205139.
5. Alexandrova V.A., Balabushevich N.G., Bondarenko G.N.,Domnina N.S., Larionova N. I. Water soluble chitosan conjugates with plant antioxidants and polyelectrolyte complexes on their basis. J. Drug Delivery Sci. Tech., 2006, v. 16 (4), p. 279-283.
6. Balkina A.S., Selischeva A.A., Sorokoumova G.M., Ollivon M., Larionova N.I.
Encapsulation of Bowman-Birk soybean proteinase inhibitor within zwitterionic phospholipid multilamellar vesicles. J. Drug Delivery Sci. Tech., 2006, v. 16 (4), p. 301-306.
7. Villemson A., Couvreur P., Gillet B., Larionova N., Gref R. Dextran-poly-ε-caprolactone micro- and nanoparticles: preparation, characterization and tamoxifen solubilization. J. Drug Delivery Sci. Tech., 2006, v. 16 (4), p. 307-313.
8. Balabushevich N.G., Lebedeva O.V., Vinogradova O.I., Larionova N. I.
Polyelectrolyte assembling for protein microencapsulation. J. Drug Delivery Sci. Tech., 2006, v. 16 (4), p. 315-319.
9. Balabushevich N.G., Sukhorukov G.B., Larionova N.I. Polyelectrolyte multilayer microspheres as carriers for bienzyme system: preparation and characterization.
Macromolecular Rapid Communications 2005, v.26, N 14, 1168-1172.
10. Tsybina T., Dunaevsky Y., Musolyamov A., Egorov T., Larionova N., Popykina N., Belozersky M. New protease inhibitors from buckwheat seeds: properties, partial amino acid sequences and possible biological role. Biol Chem. 2004, v. 385, N 5, p. 429-434.
11. Volodkin D.V., Larionova N.I., Sukhorukov G.B. Protein encapsulation via porous CaCO3 microparticles templating. Biomacromolecules. 2004, v. 5, N 5, p. 1962-1972.
12. Tiourina O.P., Antipov A.A., Sukhorukov G.B., Larionova N.I., Lvov Yu.,     Mohwald H. Encapsulation of a-chymotrypsin into the hollow polyelectrolyte microparticles. Macromolecular Bioscience 2001, v.1, p.209-214.
13. T.A. Tsybina, Y.E. Dunaevsky, N.A. Popykina, N.I. Larionova, and M.A. Belozersky, Cationic inhibitors of serine proteinases from buckwheat seeds: study of their interaction with exogenous proteinases, Biochemistry (Moscow) 2004, v.69, N 4, p. 441-444.
14. Villemson A.L., Kuskov A.N., Shtilman M.I., Galebskaya L.V., Ryumina E.V., Larionova N.I. Interaction of polymers aggregates based on steariyl-poly-N-vinylpyrrolidone with blood components. Biochemistry (Moscow), 2004, v.69, N 8, p.765-775.
15. N.G. Balabushevich and N.I. Larionova, Fabrication and Characterization of Polyelectrolyte Microparticles with Protein. Biochemistry (Moscow), 2004, v.69, N 7, с. 757-762.
16. N.G. Balabushevich, E.P. Zimina and N.I. Larionova, Encapsulation of Catalase in Polyelectrolyte Microspheres Composed of Melamine Formaldehyde, Dextran Sulfate, and Protamine. Biochemistry (Moscow), 2004, v.69, N 7, с. 763-769.
17. Volodkin D.V., Larionova N.I., Sukhorukov G.B. Protein encapsulation via porous CaCO3 microparticles templating. Biomacromolecules. 2004, v. 5, N 5, p. 1962-72.
18. Tsybina T., Dunaevsky Y., Musolyamov A., Egorov T., Larionova N., Popykina N., Belozersky M. New protease inhibitors from buckwheat seeds: properties, partial amino acid sequences and possible biological role. Biol. Chem. 2004, v. 385, N 5, p. 429-34.
19. Tiourina O., Sharf T., Balkina A., Ollivon M., Selischeva A., Sorokoumova G., Larionova N. Interaction of the water-soluble protein aprotinin with liposomes: gel-filtration, turbidity studies and 31P NMR studies.    J Liposome Research 2003, v. 13, N 3&4, p. 213-229.
20. Larionova N.V., Malykh E.V., Villemson A.L., Krasota A. Ju., Duchene D., Ollivon M., Gernet M., Belousova R.V., Shen W.-C., Larionova N.I. Effect of membranotropic and mucoadhesive formulations of protein proteinase inhibitors on bovine herpes virus-1 reproduction. Int. J. Pharm. 2003, v. 256, N 1-2, p. 191-198.
21. A. L. Villemson, E. V. Malykh, M. I. Shtilman, and N. I. Larionova, Self-Assembling Systems Based on Amphiphilic Poly-N-vinylpyrrolidone and Their Interaction with Model Proteins. Biochemistry (Moscow), 2003, v.68, N 8, p.869-874.
22. Gladysheva I.P., Popykina N.A., Zamilodchikovaa T.S., Larinova N.I. Study on interaction between doudenase-protease with dual specificity and inhibitors of Bowman-Birk family. Protein and Peptide Letters, 2002, v. 9, N 2, p.139- 144.
23. Krasota A., Belousova R., Duchen D., Larionova N. In vitro inhibition of bovine herpes virus 1 reproduction and microencapsulated proteinase inhibitor aprotinin. Journal of Controlled Release. 2002, v. 85, p. 117-124.
24. Gladysheva I.P., Moroz N.A., Karmakova T.A., Nemtsova E.R., Yakubovskaya R.I., Larinova N.I. Immunoconjgates of soybean Bowman-Birk protese inhibitor as targeted antitumor polymeric agents. Journal of Drug Targeting, 2001, v. 9, p. 303-316.
25. Tiourina O.P., Antipov A.A., Sukhorukov G.B., Larionova N.I., Lvov Yu.,     Mohwald H. Encapsulation of a-chymotrypsin into the hollow polyelectrolyte microparticles. Macromolecular Bioscience 2001, v.1, p.209-214.
26. Gladysheva I.P., Polekhina O.V., Karmakova T.A., Nemtsova E.R., Yakubovskaya R.I., Shen W.-C., Kennedy A.R., Larinova N.I. Potential of block copolymer- and immuno-conjugates for tumor-targeter delivery of Bowman-Birk soybean proteinase inhibitor. Journal of Controlled Release. 2001, v. 74, p. 303-308.
27. Balabushevitch N.G., Sukhorukov G.B., Moroz N.A., Kazanskaya, N.F., Larionova N.I., Volodkin D.V., Donath E., Mohwald H. Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release. Biotechnology and Bioengineering. 2001, v. 76, N 3, p. 207-213.
28. Larionova N.I., Moroz N.A., Tyurina O.P. Molecular design, characterization and pharmacological activity of conjugates of protein proteinase inhibitors. S.T.P. Pharma Sciences, 1999, v. 9, N 1, с. 65-80.
29. Larionova N.V., Ponchel G., Duchene D., Larionova N.I. Biodegradable cross-linked starch/protein microcapsules containing proteinase inhibitor for oral protein administration Int. J. Pharm. 1999, v. 189, N 2, p. 171- 178.

DEPARTMENT OF CHEMISTRY DIVISION OF CHEMICAL ENZYMOLOGY
Main page	Prof. Lariononva N.I. Head of the research group, leading scientist e-mail: nilar(at)enzyme.chem.msu.ru Larionova N.I., Doctor of Philosophy since 1971, Doctor of Science since 1991, Professor since 2001, has over 200 scientific publications in Russian and International Journals. Larionova N.I. actively cooperates with foreign colleagues and has joint publications with scientists from the USA, France, Germany & Cuba. Hirsch index that quantifies both the actual scientific productivity and the apparent scientific impact of a scientist is 12. Larionova N.I. has founded a novel trend at Chemistry department in immobilized proteins usage in developing prolonged & targeted protein delivery systems. The scientific group headed by Larionova N.I. has completed a number of major studies in kinetics & mechanism of highly specific protein-protein interactions; structure and functioning of cathepsin G-like proteinases; molecular design of protein inhibitor conjugates; encapsulation of proteins, enzymes & poorly soluble anticancer agents; construction of structural elements for the endo-cardial electrode pacemakers containing dexamethasone. Scientific developments of Larionova N.I. are protected by 10 USSR Author's Certificates, 4 Russian, 2 foreign & 1 international PCT Patents. For the last 10 years 17 grants including RFBS, NATO, The Ministry of Education and Science of the Russian Federation were awarded the research group. Larionova N.I. is a member of Controlled Release Society, Bioencapsulation Research Group, the Board of Trustees & the Council of Experts of I.V.Berezin Foundation. Larionova N.I. is a reviewer for Boichemistry Moscow, Biochimia et Biophysica Acta, J. Nanosci. Nanotech., Current Medicinal Chemistry, J. Pharmacy and Pharmacology, member of the Editorial Boards of J. Drug Delivery Science and Technology, Acta Pharmaceutica Sciencia. In 1999 Larionova N.I. was the Co-chairman of the Organizing Committee of an international symposium "Lipid and Surfactant Dispersed Systems". N.I.Larionova pays much attention to work with undergraduate & postgraduate students. 4 students and 2 PhD-students were awarded I.V.Berezin prize for their works carried out under her supervision. Larionova N.I. is the author of 6 manuals. 29 students and 9 PhD-students have completed their degrees under the supervision of N.I.Larionova (see section "Graduates"). Selected articles: 1. Balabushevich N.G., Larionоva N.I. Protein-loaded microspheres prepared by sequential adsorption of dextran sulphate and protamine on melamine formaldehyde core. Journal of Microencapsulation. DOI: 10.1080/02652040802518145 (в печати). 2. Larionova N.I., Zubaerova D.K., Guranda D.T.,. Pechyonkin M.A., Balabushevich N.G. Colorimetric assay of chitosan in presence of proteins and polyelectrolytes by using o-phthalaldehyde. Carbohyd. Polym., 2009, v. 75, N 4, p 724-727. 3. Zubaerova D.Kh., Larionova N.I. Noninvasive Insulin Delivery Systems. Biomed Khim. 2008, v. 54, N 3, 346-355. 4. Kuskov A.N., Villemson A.L., Shtilman M.I., Larionova N.I., Tsatsakis A.M., Tsikalas I., Rizos A.K. Amphiphilic poly-N-vinylpyrrolidone nanocarriers with incorporated model proteins. J. Phys-Condens. Mat., 2007, v. 19, N 20, Article number 205139. 5. Alexandrova V.A., Balabushevich N.G., Bondarenko G.N.,Domnina N.S., Larionova N. I. Water soluble chitosan conjugates with plant antioxidants and polyelectrolyte complexes on their basis. J. Drug Delivery Sci. Tech., 2006, v. 16 (4), p. 279-283. 6. Balkina A.S., Selischeva A.A., Sorokoumova G.M., Ollivon M., Larionova N.I. Encapsulation of Bowman-Birk soybean proteinase inhibitor within zwitterionic phospholipid multilamellar vesicles. J. Drug Delivery Sci. Tech., 2006, v. 16 (4), p. 301-306. 7. Villemson A., Couvreur P., Gillet B., Larionova N., Gref R. Dextran-poly-ε-caprolactone micro- and nanoparticles: preparation, characterization and tamoxifen solubilization. J. Drug Delivery Sci. Tech., 2006, v. 16 (4), p. 307-313. 8. Balabushevich N.G., Lebedeva O.V., Vinogradova O.I., Larionova N. I. Polyelectrolyte assembling for protein microencapsulation. J. Drug Delivery Sci. Tech., 2006, v. 16 (4), p. 315-319. 9. Balabushevich N.G., Sukhorukov G.B., Larionova N.I. Polyelectrolyte multilayer microspheres as carriers for bienzyme system: preparation and characterization. Macromolecular Rapid Communications 2005, v.26, N 14, 1168-1172. 10. Tsybina T., Dunaevsky Y., Musolyamov A., Egorov T., Larionova N., Popykina N., Belozersky M. New protease inhibitors from buckwheat seeds: properties, partial amino acid sequences and possible biological role. Biol Chem. 2004, v. 385, N 5, p. 429-434. 11. Volodkin D.V., Larionova N.I., Sukhorukov G.B. Protein encapsulation via porous CaCO3 microparticles templating. Biomacromolecules. 2004, v. 5, N 5, p. 1962-1972. 12. Tiourina O.P., Antipov A.A., Sukhorukov G.B., Larionova N.I., Lvov Yu., Mohwald H. Encapsulation of a-chymotrypsin into the hollow polyelectrolyte microparticles. Macromolecular Bioscience 2001, v.1, p.209-214. 13. T.A. Tsybina, Y.E. Dunaevsky, N.A. Popykina, N.I. Larionova, and M.A. Belozersky, Cationic inhibitors of serine proteinases from buckwheat seeds: study of their interaction with exogenous proteinases, Biochemistry (Moscow) 2004, v.69, N 4, p. 441-444. 14. Villemson A.L., Kuskov A.N., Shtilman M.I., Galebskaya L.V., Ryumina E.V., Larionova N.I. Interaction of polymers aggregates based on steariyl-poly-N-vinylpyrrolidone with blood components. Biochemistry (Moscow), 2004, v.69, N 8, p.765-775. 15. N.G. Balabushevich and N.I. Larionova, Fabrication and Characterization of Polyelectrolyte Microparticles with Protein. Biochemistry (Moscow), 2004, v.69, N 7, с. 757-762. 16. N.G. Balabushevich, E.P. Zimina and N.I. Larionova, Encapsulation of Catalase in Polyelectrolyte Microspheres Composed of Melamine Formaldehyde, Dextran Sulfate, and Protamine. Biochemistry (Moscow), 2004, v.69, N 7, с. 763-769. 17. Volodkin D.V., Larionova N.I., Sukhorukov G.B. Protein encapsulation via porous CaCO3 microparticles templating. Biomacromolecules. 2004, v. 5, N 5, p. 1962-72. 18. Tsybina T., Dunaevsky Y., Musolyamov A., Egorov T., Larionova N., Popykina N., Belozersky M. New protease inhibitors from buckwheat seeds: properties, partial amino acid sequences and possible biological role. Biol. Chem. 2004, v. 385, N 5, p. 429-34. 19. Tiourina O., Sharf T., Balkina A., Ollivon M., Selischeva A., Sorokoumova G., Larionova N. Interaction of the water-soluble protein aprotinin with liposomes: gel-filtration, turbidity studies and 31P NMR studies. J Liposome Research 2003, v. 13, N 3&4, p. 213-229. 20. Larionova N.V., Malykh E.V., Villemson A.L., Krasota A. Ju., Duchene D., Ollivon M., Gernet M., Belousova R.V., Shen W.-C., Larionova N.I. Effect of membranotropic and mucoadhesive formulations of protein proteinase inhibitors on bovine herpes virus-1 reproduction. Int. J. Pharm. 2003, v. 256, N 1-2, p. 191-198. 21. A. L. Villemson, E. V. Malykh, M. I. Shtilman, and N. I. Larionova, Self-Assembling Systems Based on Amphiphilic Poly-N-vinylpyrrolidone and Their Interaction with Model Proteins. Biochemistry (Moscow), 2003, v.68, N 8, p.869-874. 22. Gladysheva I.P., Popykina N.A., Zamilodchikovaa T.S., Larinova N.I. Study on interaction between doudenase-protease with dual specificity and inhibitors of Bowman-Birk family. Protein and Peptide Letters, 2002, v. 9, N 2, p.139- 144. 23. Krasota A., Belousova R., Duchen D., Larionova N. In vitro inhibition of bovine herpes virus 1 reproduction and microencapsulated proteinase inhibitor aprotinin. Journal of Controlled Release. 2002, v. 85, p. 117-124. 24. Gladysheva I.P., Moroz N.A., Karmakova T.A., Nemtsova E.R., Yakubovskaya R.I., Larinova N.I. Immunoconjgates of soybean Bowman-Birk protese inhibitor as targeted antitumor polymeric agents. Journal of Drug Targeting, 2001, v. 9, p. 303-316. 25. Tiourina O.P., Antipov A.A., Sukhorukov G.B., Larionova N.I., Lvov Yu., Mohwald H. Encapsulation of a-chymotrypsin into the hollow polyelectrolyte microparticles. Macromolecular Bioscience 2001, v.1, p.209-214. 26. Gladysheva I.P., Polekhina O.V., Karmakova T.A., Nemtsova E.R., Yakubovskaya R.I., Shen W.-C., Kennedy A.R., Larinova N.I. Potential of block copolymer- and immuno-conjugates for tumor-targeter delivery of Bowman-Birk soybean proteinase inhibitor. Journal of Controlled Release. 2001, v. 74, p. 303-308. 27. Balabushevitch N.G., Sukhorukov G.B., Moroz N.A., Kazanskaya, N.F., Larionova N.I., Volodkin D.V., Donath E., Mohwald H. Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release. Biotechnology and Bioengineering. 2001, v. 76, N 3, p. 207-213. 28. Larionova N.I., Moroz N.A., Tyurina O.P. Molecular design, characterization and pharmacological activity of conjugates of protein proteinase inhibitors. S.T.P. Pharma Sciences, 1999, v. 9, N 1, с. 65-80. 29. Larionova N.V., Ponchel G., Duchene D., Larionova N.I. Biodegradable cross-linked starch/protein microcapsules containing proteinase inhibitor for oral protein administration Int. J. Pharm. 1999, v. 189, N 2, p. 171- 178.
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