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Кодировка:
Tetrahedron: Asymmetry : Synthesis of chiral epsilon-lactones in a two-enzyme system of cyclohexanone mono-oxygenase and formate dehydrogenase with integrated bubble-free aeration  
Tetrahedron: Asymmetry
Volume 8, Issue 15 Abstract
14 August 1997 Journal Format-PDF (194 K)
Pages 2523-2526 

PII: S0957-4166(97)00311-X

Synthesis of chiral -lactones in a two-enzyme system of cyclohexanone mono-oxygenase and formate dehydrogenase with integrated bubble-free aeration

Sebastian Rissoma, Ulrich Schwarz-Linekb, Marina Vogelb, Vladimir I. Tishkovc and Udo Kragla, *

a Institut fЭr Biotechnologie, Forschungszentrum JЭlich GmbH D-52425 JЭlich Germany
b Institut fЭr Organische Chemie, UniversitДt Leipzig, Talstraъe 35 D-04103 Leipzig Germany
c Department of Chemical Enzymology, M. V. Lomonosov Moscow State University RUS-119899 Moscow Russia

Received 20 June 1997. Available online 21 April 1998.

Abstract

A two-enzyme system consisting of a cyclohexanone mono-oxygenase from Acinetobacter NCIMB 9871 and a protein-engineered formate dehydrogenase for the regeneration of the cofactor NADPH was used for the synthesis of chiral -lactones. 4-Methylcyclohexanone was used as the model substrate yielding (S)-(-)-5-methyl-oxepane-2-one with high chemical and enantiomeric purity. Syntheses were carried out in a repetitive-batch reactor with integrated bubble-free aeration by means of a thin-walled silicon tube.

*Corresponding author.

 
Tetrahedron: Asymmetry Abstract
Journal Format-PDF (194 K)
Volume 8, Issue 15
14 August 1997 
Pages 2523-2526