Department of chromatography as scientific and methodical department of Institute provides support of chromatography stages in the investigations of macromolecules and their fragments, which are carried out at the Institute.

The main subject of our research work is studying the structural organization and functioning of biological macromolecules.

In collaboration with Semenov Institute of Chemical Physics of the RAS we developed a new tritium planigraphy method, which allows to study accessible surface and with its help to construct three dimensional structure of macromolecules and supramolecular systems.

The structural organization and expression of genome of Archaea are under investigation (Dr. V.K.Kagramanova and co-workers). The number of archaebacterial plasmids and operon of rRNA were studied.

The investigation of cell-cell contact interactions and their influence on cellular metabolism has been performed (Prof. L.B.Margolis and co-workers).

1. Goldanskii V.I., Kashirin I.A., Shishkov A.V., Baratova L.A.,Grebenchshikov N.I. The use of thermally activated tritium atoms for structural-biological investigation: the topography of the TMV protein- accessible surface of the virus. J. Mol. Biol. (1988) 201, 567-574.

2. Baratova L.A., Grebenchshikov N.I., Dobrov E.N., Gedrovich A.V., Shishkov A.V., Kashirin I.A., Efimov A.V., Jarvekulg ., Radavsky Yu.L., Saarma M. The organization of potato virus X coat proteins in virus particles studies by tritium planigraphy and model building. Virology (1992) 188, 175-180.

3. Mankin A.S., Zyryanova I.M., Kagramanova V.K., Garrett A. Introducing mutations into the single chromosomal 23S RNA gene of archaeon Halobacterium hal. using an RNA operon-based transformation system. Proc. Natl. Acad. Sci. USA (1992) 89, 6535-6538.

4. Galkina S.I., Sudўina G.F., Margolis L.B. Regulation of intracellular pH by phospholipase A2 and protein kinase C upon neutrophil adhesion to solid substrata. FEBS Letters (1996) 393, 117-120.

5. Bogacheva E.N., Goldanskii V.I., Shishkov A.V., Galkin A.V., Baratova L.A., Tritium planigraphy: from accessible surface to protein spatial structure. Proc. Natl. Acad. Sci. USA (1998) 95, 2790-2794.