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Кодировка:

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6. J. . Dekker and . J. Boekema, Biochim. Biophys. Acta 1706, 12 (2005). 7. . Shimoni, . Rav-Hon, 1. Ohad, et al., Plant ll 17, 40 2580 (2005). 8 8. J. . Cruz, . . Salbilla, . , and . . . m, Plant Physiol. 127, 1167 (2001). 9. W. Haehnel, . , and . Krause, Biochim. . Biophys. Acta 593, 384 (1980). 10. W. Haehnel, R. Ratajczak, and . Robenek, J. ll if- - Biol. 108, 1397 (1989). , . G. . , E.-W. , and N. . Kostic, J. m. m. Soc. 119, 42 (1997). 12. . . Pearson Jr. and . L. Gross, Biophys. J. 75, , 2698 (1998). . , 13. F. Rienzo, R. Gabdoulline, . Menziani, . al., Bio phys. J. 81, 3090 (2001). 14. . L. Gross and . . Pearson Jr., Biophys. J. 85, 2055 (2003). , 15. . . , . . , . . ., 48 (4), 656 (2003). 16. 1. . Kovalenko, . . Abaturova, . . Gromov, et l., Phys. Biol. 3, 121 (2006). , 17. L. . Staehelin . W. . van der Staay, in Oxygenic Photosynthesis. The Light Reactions, Ed. . R. Ort and . F. m (luwer Academic PubIishers, 1996), [25]. . 11-30. (!) 18. .-. Albertsson, Trends in Plant Science 6 (8), 349 , (2003). 19. . Stroebel, . Choquet, J.-L. Popot, and . Picot, . Nature 426, 413 (2003). , 20. . Ubbink, . Ejdebeck, . . Karlsson, and . S. , Bendall, Structure 6, 323 (1998). 21. . Kleanthous, Protein-protein recognition (Oxford Uni f . versity Press, N ew York, 2000). 22. . . . . , . ( «», ., 2002). 23. . Kaant, S. Young, and . S. Bendall, Biochim. 1. . . , Biochim. Biophys. Acta 1143, 1 (1993). Biophys. Acta 1277, 115 (1996). 2. . L. Gross, in Oxygenic photosynthesis: the light reactions, 24. . . , Ed. . R. Ort and . F. m (Kluwer Acad. (- , ., 2003). ., Dordrecht, 1996), . 413-429. 25. . . , ( « 3. . , . Sarafis, and . Critchley, Aust. J. Plant », ., 2000). Physiol. 26, 709 (1999). 26. . . , . . , . . 4. . . , Biochim. Biophys. 1456, 5 (2000). ., . . 12 (3), 955 (2005). 5. S. Murakami and L. , J. ll Biol. 47, 332 (1970).

53

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2008

~70

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Computer Simulation ! Complex Formation "between Plastocyanine and Cytochrome f in Thylakoid Lumen
I.B. Kovalenko*, .. Aaturova*, .. Gromov, D.M. Ustinin, G.Yu. znink, .. Gracbev*' a';d .: Rubin
*ioZogicaZ

Department, Lomonosov Moscow State University, Vorob'evy Gory, Moscow, 119992 Russia

**PhysicaZ Department, Lomonosov MoscowState University, Vorob'evy Gory, Moscow, 119992 Russia
diffusion of the protein plastocyanine and complex formation between plastocyanine and cytochrome j ( subunit of cytochrome I! complex) in the chloroplast thylakoid lumen has studied. 3D computer simulation model of diffusion and binding of plastocyanine and cytochrome j has constructed, which considers their electrostatic interaction. Based the experimental data, the parameters of the model for complex formation between plastocyanine and cytochromejin solution have estimated. dependence ofthe rate ofplastocyanine-cytochrome j reaction the size of the luminal space has studied. It was shown that the contraction of the luminal space leads to decrease in the reaction , which is in agreement with the experimental data the inhibition of the reaction under hyperosmotic stress.

words: pZastocyanine, cytochrome f, difjusion, protein compZex,

,

eZectrostatic interactions

53

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2008