Äîêóìåíò âçÿò èç êýøà ïîèñêîâîé ìàøèíû. Àäðåñ îðèãèíàëüíîãî äîêóìåíòà : http://www.sevin.ru/dissertations/ecology/107.pdf Äàòà èçìåíåíèÿ: Mon Oct 28 15:32:58 2013 Äàòà èíäåêñèðîâàíèÿ: Fri Feb 28 18:51:09 2014 Êîäèðîâêà:

FELISCATUS
03.02.04-



2013


. .. , : , . .. . : , , . .. . , , . .. . :

26 2013 14 002.213.01 . .. 119071, . , ., .33. ./ 8(495)9527324, email: admin@sevin.ru 119071, . , ., .33. 25 2013 . ...
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. , , , (,1986). , , « ­ ». (Wyatt, 2012). , («alarm pheromones») , , . , , , , . (TAARs), , , , (Liberles, Buck, 2006). , TAARs (« »), , (Johnson et al., 2012). . 14 TAARs4 ­ 2-, 38 (Ferrero et al., 2011). « » , , , , ,
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. (. Apfelbach et al., 2005). 2013 , (Osada et al., 2013; Starke and Ferkin, 2013; Voznessenskaya, 2013). . , , , , , . Mus musculus Felis catus. , . , «» ­ . L- (, 3--3--1-, ), , , : (Miyazaki et al., 2006; Miyazaki, 2008). L- , , , , .. L- , ; , (Miyazaki et al., 2008). L-, , L- . - L- - Mus musculus Rattus norvegicus, , , .

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Felis catus : Rattus norvegicus Mus musculus. L . L- . . L- . , L- , , . , L- , (0,05%), ; ; . , L- , (0,05%), , . , L- . , L- . , L- (5-3,11-diol) . , L- (3--3--1-, ) , ().
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. , (L- ), Felis catus, , (Mus musculus, Rattus norvegicus) : . (L-) . . , , , . , L- , / . : , . , : 1. ( L-) ; Mus musculus. 2. L- , Mus musculus. 3. Felis catus , , Mus musculus. 4. L- , : - - Felis catus.
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17 2013 . : XVI International Symposium on Olfaction and Taste (ISOT)2012 ., , ; International Conference of Association for Chemoreception Science (AChemS) 2011., , ; Congress of European Chemoreception Research Organization (ECRO) 2010 ., , ; 2011, , ; 2013., , ; XII International conference "Chemical Signals in Vertebrates" 2011 ., , ; - « XXI » 2011 ., 2013., ; 7- " " 2011 ., , , ; 7- «: » 2013 ., . , ( , , , ), , , . 131 , 35 3 ; 412 , 394 . 1. : «- »; « »; « , ». 2. . Musmusculus Rattus norvegicus 2-6 . , , , : C57BL/6J, CBA/Lac-Sto, BAL/. , , . 12/12 ­ 22-24° , («», ) .
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. : , . . . . . Felis catus, . L- (US Biological, USA) , (0,05%) (Hendriks et al., 1995). ­ Cavia porcellus . , . . . , (ASP130; ASP110, Flur France). , 14 . - 16-18 . . . : ; ; ; ; 21 . . , , , () DR G ()EIA-1559,EIA-4164 EIA-1561 , SpectraMax 340PC 384. SpectraMax Software http://www.moleculardevices.com/pages/software/softmax.html). . .
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. L- , 5-3,11-diol (Touma et al., 2004). L -20° . 80%- . . . . . ( . ). SpectraMax Software. . L- 40 , (c-fos). - . , ­ (). () () ( 30 ) Santa Cruz Biotechnology, (): -fos(4)sc-52 1:1000 Fos. . (ANOVA, t- ) ( -). «-» - 2. ± (M±SE). p<0,05 . Statistika 8.

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3. ( ) (Dick man, 1992; Kavaliers, Choleris, 2001), (Blanchard et al., 2001; Zangrossi, File, 1992; Dielenberg, McGregor, 2001). () , . 3.1. (n=16, =0,0057, ) (1).

1. (0,05 .) - (-) (n=16, **=0.0057, ), - (-) (n=16, =0,34, ).

- L- (0,05%, 0,05 .) 70% (n = 20), 20% (n=20, p=0,0018, ). L- 62,5% (n = 8) 12,5 % (n = 8, p = 0,043) ( 2).
2. L (0,05%, 0,05 .) - (n=20, **p=0,0018, ), (n=8, *p=0,043 ).

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, L-, , - (p=0,0337, n=8, - ), - ( 3).
3. L- (0,05%, 0,05 .) - (-) (*p=0,0337, n=8, - ); - () (p=0,72, n=8 - , (M±SEM), ­ ).

( 4).
4. (0,05 .) [(t=-4,8, ***p=0,00008 df=22, n()=11, n()=13; ; (M±SEM), ­ .]

- 2 , - . (Drickamer, 1991;Norrdahl, Korpimaki, 2002). - ( L- ) . , L .
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, - , - , , , - . , . , . ( L) 2,5 ± 1.00 (n = 28), ­ 5,70 ± 1,00 (n=28, p=0,046 -, ) ( 5). (p<0,05) L-, .
5. L- , (n=28 , *p=0,046, . (M±SEM), ­ ).

, , , (Harvel, 1990; YlÆnen, Ronkainen, 1994; Tollrian, Harvell, 1999; Apfelbach et al., 2005). , , , . 70 % , ­ 49% (p<0,01). L- (0,05%) 61-64 % , ­ 48-50 % ( 6). (McClure, 1981; Meikle, Dricka mer, 1986; Hendricks, McClintock, 1990; Perret, 1990; Clarc et al.,1992; Bacon, McClintock, 1994). , (Krackow, 1992),
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(Hahn, (Huck

Hays, 1963; Huck et al., 1988; Krackow, 1992). , , .. 8- et al., 1988; Krackow et al., 2003).

6. L- (0,05%, 0,05.) 1- : - -[* p=0,036, n()=35, n()=61, 2=4,393]; - [*p=0,032, n()=37, n()=69, 2= 4,56, p=0,032]. (0,05 .) 1- [**p=0,0055, n()=52, n()=118, 2 = 7,692].

, , , , (Cushing, 1985; Drickamer, 1991). 3.2. (0,2 ) ( 7).
7. (0,2 .) L- (0,05%, 0,2 .) - [(ANOVA, F(2, 36)=4,62, n=13, p=0,016, Eta= 0,20, - , **p=0,0089), (M±SEM), ].

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, L- (0,05 %, 0,2 ). L-. , (Dewsbury, Hartung, 1980; Lohmiller, Swing, 2006; Marashi, Rulicke, 2012) , . (Voznessenskaya et al., 1992;Voznessenskaya et al., 2003). , L- (0.05%), (p<0,001,n()=110, n()=113) ( 8).
8. L (0.05%, 0,2 .) - . [***p<0,001, n()=110, n()=113, 2 = 19,23].

, 70% ( 9).
9. (0,2 .) . [***p<0,001, n()=77, 2 n()=113, =12,48].

, , , , . , , .
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L- (21 ), . - (42,6±0,15.) (48±0,17 .) (p=0,005, df=143, t=-2,45, t- ) ( 10).
10. L (0.05%, 0,2 .) (21- ) , [** p=0,005 df=143, t=-2,45, , (M±SEM), ­ ].

, L- - , (p<0,001) , 12 . (p<0,001) 10 . ( 11).
11. (0,2 .) L- (0,05%, 0,2 .) (21- ) - . [ANOVA (2,298) = 25,89, ***p<0,001, - F(2,298) = 23,81, ***p=0,000022, ***p=0,000028. (M±SEM), ­ ].

, (p<0,001) . . , , (HeikkilÄ et al., 1993;Carlsen et al., 1999). ,
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(, , 2011; Bourke et al., 2013). 3.3. (15 ) (p<0,001, n=8) ( 12). 2 , 3- . , , . ( ) , ( 12).

12. ( 15 ., (1); - (2); « » - (/) () ( - ) ***p=0,000164), (M±SEM)),

0,2 .) (Felis catus) ­ (3); (4) [(ANOVA. F(3, 28)=32,12, p < 0,001, ­ ].

« ». ( 12). , ( ) , .
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L- (0,05%, 0,05 ., 10 ) ( 13), .
13. (10 ) L- (0,05%, 0,05 .) (5-3b,11-ehol) (Mus musculus) [(***p<0.001, t=-14,21, n=13 t- ), (M±SEM), ­ ].

, , , L-. , , (Broom, Johnson, 1993; Liptrap, 1993; Dobson, Smith,1995; Balm, 1999; DeCatanzaro, 2011). , . - (Zangrossi, File, 1994; Dielenberg, McGregor, 1999; Blanchard et al., 2001). , (Blanchard et al., 2001a; Apfelbach et al., 2005). «» . (Stoddart, 1980; Weldon et al., 1987; Weldon, 1990; Zimmerling, Sullivan, 1994; Apfelbach et al., 2005; Starke, Ferkin, 2013). , , . 8- , ..
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. ( 14, p<0,001, n=10, t=18,36, t- ).
14. (0,2 .) [(***p<0.001, n=10, t=18,36, t- ), (M±SEM), ­ ].

, . . (2 ) L-(0,2 , 0,05%) (p=0,049, n=10, t=2,26, t-) ( 16). L- 0,1 ( 15). (Blanchard et al.,1993;HeikkilÄ et al., 1993;Frohlich et al., 2002) ( 15).

15. ( ) (0,2 .) (**p =0,0020, n=10, t=3,604, t- ). (M±SEM), ­ .

16. L- (0,05%, 0,1 0,2 .) (*p=0,049, n=10, t=2,266, t- .(M±SEM), ­ ).
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(p=0,0020, n=10, t=-3,604), L-. . , , , , (Wang, Liu, 2002; Zhang et al., 2003). , , . 3.4. () L- L- () ( 17), () ( 18).

50µm 250µm 17. (t=40.) L (0,05%) . (n=4, ) Fos- . 18. (t=40.) (n=4, ). .

250µm
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() ( 19). ( 20) .

50 µ m 250µm 19. (t=40.) L (0,05%) . (n=4, ) Fos- .

: () () (). , , (Baxi, 2006), 250µm , 20. (Kimoto et al., 2005; Leinders-Zufall et al., (t=40.) (n=4, 2000). ). () . . , , V1R (Berghard et al., 1996; Dulac and Axel, 1995), , . V2R (Herrada and Dulac, 1997; Matsunami and Buck, 1997; Ryba and Tirindelli,1997),
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, (Krieger et al., 1999). . , Fos- , L- . Fos, L, , , , L- , , . L- . L- , ,3--3--1- (MM) . (Miyazaki et al., 2006). , , , (Voznessenskaya et al., 2006; Voznessenskaya et al., 2007). , , (), , . 1. , L- ; ; , . 2. , L- ; , . 3. L- , - .

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4. (10 ), , L, , . 5. . 6. (2 ) L- (0,05%, 0,2 ) . 7. (c-fos) , ( ) L- . 8. : L-, , L- , .

07-0401538 10-04-01599, - 709.2012.4 « ». , , ... . , . , , .

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( ) 1. .. Felis catus Mus musculus / .. , .. // . ­2013.- . 453.-N2. -.227-229. ( ) 2. .. Rattus norvegicus / .. , .. // . -2013.- 4 ( 1).-. 121-124. 3. .. [ ] /.. // . ­ 2013.1.- 8 .- URL: http://www.science-education.ru/107-8184 ( : 18.09.2013). , , ; 4. Voznessenskaya V.V. Development of nontoxic methods of rodent population control as an alternative approach for big cities /V.V. Voznessenskaya, T.V.Malanina// News in Chemistry, Biochemistry and Biotechnology: State o f the art and Prospects of Development, Nova Science Publishers, Inc., New York. ­2013 (in press). 5. Malanina T.V. The influence of Felidae family pheromone precursor L-felinine on reproductive status of the house mice /T.V. Malanina, A.B. Klinov, V.V. Voznessenskaya //High Technologies, Basic and Applied Research in Physiology and Medicine. onference proceedings. St. Petersburg.-2011.- Vol 2.- P.224-230. 6. Voznessenskaya V.V. Development of nontoxic methods of rodent population control / V.V.Voznessenskaya, A.B.Klinov, T.V.Malanina // High Technologies, Basic and Applied Research in Physiology and Medicine. onference proceedings. St. Petersburg.-2011.-Vol 2.- P.154-161. 7. .. L- / .., .. // . -2012.- 6.-.17-19. 8. .. (Rattus norvegicus) / .. // .2012.- 11.-.12-13. 9. Voznessenskaya V.V. The influence of L-Felinine on reproduction of mice and rats / V.V.Voznessenskaya, T.V. Malanina//Chemical Senses. ­2011.-1(36).P.E32. 10. Voznessenskaya V.V. L-felinine as potential reproductive inhibitor in rodents /V.V.Voznessenskaya, A.B.Klinov, T.V.Malanina//Che mical Senses. -2011.- .9(36).-P.A46.
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11. Malanina T.V. Long-term exposure to predator odors affects plasma testosterone in male rats / T.V.Malanina, V.V. Voznessenskaya // The European Chemoreception Research Organization XXIII Congress. Abstracts., Leuven, Belgium. ­2013.-P.117. 12. Voznessenskaya V.V. Gender specific responses to predator che mical cues in the house mouse: the effects of early olfactory experience. / V.V.Voznessenskaya, T.V. Malanina, A.B.Klinov, T.K.Lactionova // The European Chemoreception Research Organization XXIII Congress. Abstracts., Leuven, Belgium ­2013.-P.178. 13. .. Mus Musculus Rattus Norvegicus / .., .. // 17- - « ­ XXI ». .- 2013. -.544-545. 14. .. / .. , .. // «: ». - 2013.-.317-318. 15. .. L : / .., .., .. // « ». .-2012.-.25. 16. Malanina T.V. L-Felinine as a potential chemical signal in the house mouse / T.V.Malanina, V.V.Voznessenskaya // The European Chemoreception Research Organization XXI Congress. Abstracts., Manchester.UK.-2011.-P.113. 17. Voznessenskaya V.V. L-felinine as a Putative Pheromone in the House Mouse /V.V.Voznessenskaya, T.V.Malanina // Abstracts of Seventh International Interdisciplinary Congress «Neuroscience for Medicine and Psychology». Sudak, Crimea, Ukraine. -2011.-P.116. 18. Malanina .V. Neural Activity in Main Olfactory and Accessory Olfactory System of the House Mouse in Response to Stimulation with L-felinine /.V.Malanina, .B.Klinov, V.V.Voznessenskaya //Abstracts of Seventh International Interdisciplinary Congress «Neuroscience for Medicine and Psychology». Sudak, Crimea, Ukraine.-2011.- P.279. 19. Klinov A.B. The influence of L-felinine on reproduction in the house muse /A.B.Klinov, T.V.Malanina, V.V.Voznessenskaya // Chemical Signals in Vertebrates XII. Abstracts., Berlin, Germany.-2011.- P.106. 20. .. L- / .., .. // 15- - « ­ XXI » . -2011.-.174.

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