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Plecotus et al. 17 (2014): 18­29 _____________________________________________________________________________

Myotis dasycneme (Boie, 1825),
.. 1, 2, .. 1, .. 3, .. 1, .. 1
1

, . 8 , 202, 620144; kovalchuk@ipae.uran 2 . .. , . , 19, 620002; innamoramento23@yandex.ru 3 , -1, 456317; snitko@ilmeny.ac.ru - . Myotis dasycneme . . , . : Myotis dasycneme, , , , ,

, . , (Agreement... 1991; The Convention... 1992), (Boye, Dietz 2005). ( 1999; 2000; . 2005; 2013), . , , , . Myotis dasycneme ­ -


Plecotus et al. 17 (2014)

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. , (

, , 1998 1730 . 2005). ( 2001).

40 M. dasycneme. 2013 ( ) 21­23º ­ 5­7º. ­ ( 1999). M. dasycneme. , ( ... 1986). BC­5800 (Mindrai, China) 10 : (WBC, /=109 /), (RBC, /=1012 /), (HGB, /), (HCT, %), (MCV, ­ =10-15 ), (MCH, ­ =10-12 ), (MCHC, /), (PLT, /), (MPV, ), (PCT, %). ( ) , -. 100 . ( 2004). "Statistica for Windows 6.0". U- -. p<0.05, p<0.01. -


20 . , , (MCV, MCH, MCHC, MPV) , - (. 1). : 187.9±3.2 / , 177.6±2.1 ­ (p=0.01) : 1.9±0.1 / , 4.7±0.5 (p=0.01). , . , , - , . , . , (Rishpon-Meyerstein et al. 1968; Ferucci et al. 2006).
1. Myotis dasycneme Table 1. Indicators of peripheral blood in adult Myotis dasycneme (WBC), / (RBC), / (HGB), / (HCT), % (MCV), (MCH), (MCHC), / (n=10) ±SE (min­max) 1.9±0.1 (1.5­2.3) 11.3±0.5 (9.0­12.9) 187.9±3.2 (177.0­200.0) 53.5±1.7 (45.5­9.3) 47.6±0.4 (45.7­49.1) 17.3±0.7 (15.2­19.6) 357.0±9.9 (331.0­394.0) 319.6±20.9 (267.0­368.0) 6.3±0.2 (5.7­7.1) 0.1±0.02 (0.1­0.2) (n=8) ±SE (min­max) 4.7±0.5* (3.9­6.0) 11.1±0.2 (10.6­11.6) 177.6±2.1* (175.0­186.0) 51.17±1.05 (46.3­54.0) 46.7±0.3 (45.9­47.7) 15.6±0.2 (14.8­16.2) 341.3±3.5 (326.0­361.0) 295.2±17.2 (253.0­342.0) 6.6±0.1 (5.9­7.1) 0.2±0.01 (0.1­0.2)

(PLT), / (MPV), (PCT), %

: * ­ (p<0.05)


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, ( . 2010; 2013). ( 2.5 , , p=0.01), . (p=0.004), (p=0.01) (. 1). , , , ( . 2004). : 1.0±0.1 / 1.8±0.2 p=0.02. . , . M. dasycneme ­ ( 1.8 , , p=0.006) ( 2.1 , p=0.03), (. 1). , (1.1% 4.6% , p=0.001), (. 1). , . (, 2002). M. dasycneme. (. 1). . (adultus, subadultus) (RBC, HGB, HCT, MCV) (. 2). 1.6 , ( p=0.01). (, 2011).


22 48.5±0.8 (52.3±0.6, p=0.01). , , ( 2012).

. 1. Myotis dasycneme. * ­ (p<0.05) Fig. 1. Leukocytic pool of peripheral blood in male s and females of Myotis dasycneme. * ­ differences between the groups are statistically significant (p<0.05)


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, (152.6±5.5 101 .5±6.5 , p=0.01). HGB subadultus , , .
2. Myotis dasycneme Table 2. Age variation of peripheral blood indicators in female Myotis dasycneme adultus (n=5) subadultus (n=4) ±SE ±SE (min­max) (min­max) 2.9±0.4 2.7±0.3 (WBC), / (2.04­3.9) (2.1­3.1) 9.3±0.5 5.7±0.4* (RBC), / (8.24­10.9) (4.6­6.5) 152.6±5.5 101.5±6.5* (HGB), / (136.0­166.0) (83.0­111.0) 45.1±1.8 30.0±2.3* (HCT), % (41.9­50.2) (23.4­33.6) 48.5±0.8 52.3±0.6* (MCV), (46.3­50.9) (51.5­54.0) 16.6±1.1 17.8±0.2 (MCH), (12.5­18.5) (17.2­18.2) 358.8±9.4 341.0±5.4 (MCHC), / (341.0­377.0) (330.0­355.0) 112.8±24.2 213.0±32.3* (PLT), / (63.0­179.0) (158.0­293.0) 6.0±0.3 6.6±0.2 (MPV), (5.0­6.8) (6.2­6.9) 0.1±0.02 0.1±0.02 (PCT), % (0.03­0.12) (0.1­0.19) : * ­ (p<0.05)

1.5 (p=0.01). ­ 1.9 (p=0.01). , , , ( . 2005). , (. 2). -


24 . .

. 2. Myotis dasycneme Fig. 2. Age patterns of leukocytic pool of peripheral blood in female s of Myotis dasycneme


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, , , (. 3).
3. Myotis dasycneme Table 3. Seasonal changes in hematological indicators of peripheral blood in females of Myotis dasycneme (n=5) ±SE (min­max) 2.9±0.4 (2.0­3.9) 9.3±0.5 (8.24­10.9) 152.6±5.47 (136.0­166.0) 45.1±1.8 (41.9­50.2) 48.5±0.8 (46.3­50.9) 16.6±1.1 (12.5­18.5) 358.8±9.4 (341.0­377.0) (n=8) ±SE (min­max) 4.7±0.5* (3.9­6.0) 11.1±0.2* (10.6­11.6) 177.6±2.1* (175.0­186.0) 51.2±1.05* (46.3­54) 46.7±0.3* (45.9­47.7) 15.6±0.2 (14.8­162) 341.3±3.5 (326.0­361.0) P-value (U-test MannWhitney) 0.04 0.02 0.01 0.03 0.03 0.08 0.09 0.01 0.16 0.01 <0.05)



(WBC), / (RBC), / (HGB), / (HCT), % (MCV), (MCH), (MCHC), / (PLT), /

112.8±24.2 295.2±17.2* (63.0­179.0) (253.0­342.0) 6.0±0.3 6.6±0.1 (MPV), (5.0­6.8) (5.9­7.1) 0.1±0.02 0.2±0.01* (PCT), % (0.03­0.12) (0.1­0.2) : * ­ (p

MCV 3.7% (p=0.03) . 2.6 (p=0.01) , (. 3). ( PT) , .


26 . , , , ( 2001).

. 3. Myotis dasycneme. * ­ (p<0,05) Fig. 3. Seasonal dynamics of leukocytic formula in Myotis dasycneme. * ­ differences between the groups are statistically significant (p<0.05)


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: 1.6 , (. 3). , , , (, 2010). , (. 3). , , ( , 1.6 , p=0.02 p=0.01 ), (, 2011). ( 2.8 2.2 , p=0.02 p=0.03), , , ( . 1983; 2006; 2012). , , , (. 3). , (6­7 ).
.., .. 2010. . ­ 3 (93): 44­49. .., .., .., .., .., .. 2010. ( 1). ­ 2: 55­60. .., .., .. 2005. . , , 176 . .. 2006. . , . --, - , 256 . .., . ., . . 1983. . , - , 293 . .. 2013. . ­ . . .. 4: 9­11.


28
, (, 18 1986 ). . ., . . 2002. . ., , 280 . . . 2004 - . ., -, 920 . .. 2012. . ­ 4: 276. .., .., .. 2005. . ­ 16: 38­47. .. 2000. , (Chiroptera: Vespertilionidae) . ­ 1: 89­93. .. 2013. - . . . . , . , 46 . .. 2001. (Chiroptera) . ­ Plecotus et al. 4: 69­74. .. 1999. (Chiroptera, Vespertilionidae) . ­ . . 78(12): 1441­1454. .., .. 2011. . ­ 1: 138­144. .., .., .., .. 2004. . ­ . 3: 40­41. .. 2001. . . . .-, - , - , 446 . Agreement on the conservation of populations of European bats. 1991. EURUBATS. Boye P., Dietz M. 2005. Development of good practice guidelines for woodland management for bats. ­ English Nature Research Reports 661. Commissioned by: The Bat Conservation Trust, 90 pp. The Convention on Biological Diversity. 1992. Ferucci L., Maggio M., Bandinelly S., Basaria S., Lauretani F., Ble A., Valenti G., Ershler W.B., Guralinik J.M., Longo D.L. 2006. Low testosterone levels and the risk of anemia in older men and women. ­ Arch Intern. Med. 166(13): 1380­ 1388. Rishpon-Meyerstein N., Kilbridge T., Simone J., Fried W. 1968. The effect of testosterone on erythropoietin levels in anemic patients. ­ Blood 3(4): 453­460.


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SUMMARY
Mischenko V.A., Kovalchuk L.A., Snitko V.P., Chernaya L.V., Chibiryak M.V. 2014. Blood system of the pond bat Myotis dasycneme (Boie, 1825) inhabiting the Urals. ­ Plecotus et al. 17: 18­29. The possibility and necessity of the study of blood system in bats for solving ecologo-physiological problems are substantiated. The work is devoted to the investigation of blood system in Myotis dasycneme, which is the most numerous bat species in the Urals. The experimental group consisted of 40 pond bats. Ten hematologic parameters were determined: content of white blood cells (WBC), content of red blood cells (RBC), concentration of hemoglobin in whole blood (HGB), hematocrit (HCT), mean volume of erythrocyte (MCV), mean content of hemoglobin in single erythro cyte (MCH), mean concentration of hemoglobin in erythrocytes (MCHC), contents of platelets (PLT), mean platelet volume (MPV) and trombocrit (PCT). There are no significant differences between adult males and females on main parameters of peripheral blood, except for hemoglobin and leukocytes (Table 1). Detected sex differences in leukocytic pool (Fig. 1) indicate the higher adaptive capacity of females. While comparing adult and subadult females, we found sufficient differences between them in erythropoiesis (RBC, HGB, HCT, MCV ­ see Table 2). However, the leukocytic chain remained relatively stable (Fig. 2). Remarkable changes are traced in hematologic parameters of bats caught in the fall during preparation for long hibernation (6­7 months). The erythrocytic pool shifts towards considerable increase in RBC, HGB and HCT and reducing MCV (Table 3). Leukocytosis was observed as well (Fig. 3). The results of our study suggest a good lability of sanguification in bats that allows them to respond adequately to seasonal environmental conditions. K e y w o r d s : Myotis dasycneme, system of blood, peripheral blood, sex, age, season