... Catalytic properties and stability of recombinant tobacco peroxidase with amino acid change Ile37Me . Abstract The Ile37Met substitution was generated in tobacco anionic peroxidase (TOP) by site-directed mutagenesis to mimic the soybean peroxidase (SBP), in which Met37 is responsible for the increased thermal stability. ... The expression level of the constructed enzyme was approximately 40% of the total E. coli protein. ... Moscow University Chemistry Bulletin . 2014, Vol. ... 2, P. 106 . ...