... Shape and size of leaf blades and pollen grains are important diagnostical characters in many plant groups. ... We made quantitative investigations of changes of shape and size of fresh leaf blades during herbarization and also of pollen grains after acetolysis and in fine vacuum during SEM for Nymphaea species in the European Russia. We found out that during herbarization the length of the leaf blade decreases in 1,05-1,4 times and its width decreases in 1,15-1,5 times. ...