... intrinsic fluorescence, diffuse reflectance and NIR spectroscopic imaging ? are combined as related to a multimodal approach in cardiovascular research and diagnostics. ... Both Kubelka-Munk and photon migration (PM) approaches theoretically describe diffuse reflectance, reabsorption and emission tracks in the tissue and were employed to support the reabsorption effect. ... Diffuse reflectance of a beating heart either in vivo or ex-vivo is monochromatically collected by CCD camera. ...