... Characteristic changes in tryptophan fluorescence confirm that the peptide associates with dioleoylphosphatidylcholine (DOPC) liposomes, and the binding increases drastically when liposomes contain negatively charged lipids. According to circular dichroism data, Ltc1 adopts an alpha-helical structure in membrane-mimetic environment, viz sodium dodecyl sulfate micelles and DOPC liposomes. Ltc1-induced calcein leakage from DOPC liposomes proves that the peptide is a membrane-acting agent. ...