... Among the protein structural/functional modules, short (of 3 to 10 amino acid residues) linear peptide motifs of proteins have been shown to participate in receptor binding (RGD), secretion and transportation of proteins from and to endoplasmic reticulum (KDEL and HDEL), SH3-domain-binding and cell signaling (PXXP motif) and so on [3, 4]. ... Additionally, all PSGs contain integrin-binding tripeptide RGD (residues 127-129). ... Asterisks show amino acid residues that participate in receptor binding....

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... HMW) - (-NmTm), (-NmTm w.t.) -NmTm, R91G (-NmTm R91G). ... NmTm w.t. ( ) -NmTm R91G ( ), 222 (). 1 /, ґ 0,1 /. : 10 - (pH 7.3), 100 mM NaCl, 1 mM MgCl2 1 mM -ME. ... 46 F-, 70 , 30 mM Hepes, 100 mM KCl, 1 mM MgCl2 1 mM -ME, pH 7,3. 1 /. o 50 42 -NmTm WT (Tdiss = 37,2 C) -NmTm R91G (Tdiss = 30,3 C) o o , % 100 80 60 40 20 0 20 25 30 35 40 o 45 50 , C . ... SkTm WT -SkTm R91G NmTm -SkTm, . ... SkTm w.t. ( ) -SkTm R91G ( ), 222 (). 1 /, ґ 0,1 /. : 10 - (pH 7.3), 100 mM NaCl, 1 mM MgCl2 1 mM -ME. ...