Network Working Group R. Fielding Request for Comments: 2068 UC Irvine Category: Standards Track J. Gettys J. Mogul DEC H. Frystyk T. Berners-Lee MIT/LCS January 1997 Hypertext Transfer Protocol -- HTTP/1.1 Status of this Memo This document specifies an Internet standards track protocol for the Internet community, and requests discussion and suggestions for improvements. Please refer to the current edition of the "Internet Official Protocol Standards" (STD 1) for the standardization state and status of this

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... HMW) - (-NmTm), (-NmTm w.t.) -NmTm, R91G (-NmTm R91G). ... NmTm w.t. ( ) -NmTm R91G ( ), 222 (). 1 /, ґ 0,1 /. : 10 - (pH 7.3), 100 mM NaCl, 1 mM MgCl2 1 mM -ME. ... 46 F-, 70 , 30 mM Hepes, 100 mM KCl, 1 mM MgCl2 1 mM -ME, pH 7,3. 1 /. o 50 42 -NmTm WT (Tdiss = 37,2 C) -NmTm R91G (Tdiss = 30,3 C) o o , % 100 80 60 40 20 0 20 25 30 35 40 o 45 50 , C . ... SkTm WT -SkTm R91G NmTm -SkTm, . ... SkTm w.t. ( ) -SkTm R91G ( ), 222 (). 1 /, ґ 0,1 /. : 10 - (pH 7.3), 100 mM NaCl, 1 mM MgCl2 1 mM -ME. ...