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21. Hydrophobic plant antioxidants. Preparation of nanoparticles and their
... Hydrophobic plant antioxidants. Preparation of nanoparticles and their application for prevention of neurodegenerative diseases. Review and experimental data. ... This paper summarizes the review of our own works in this field and new experimental observations on the antioxidant action of some hydrophobic plant compounds. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25478884 -- 4.4 Кб -- 09.04.2016
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22. Quaternary structure of human small heat shock protein HSPB6 (Hsp20) in crowded
... Quaternary structure of human small heat shock protein HSPB6 (Hsp20) in crowded media modeled by trimethylamine N-oxide (TMAO): Effect of protein phosphorylation. ... Effect of trimethylamine N-oxide (TMAO), well-known osmolyte, widely used to imitate crowded intracellular conditions, on the quaternary structure of recombinant human small heat shock protein HspB6 (Hsp20) was analyzed by means of size-exclusion chromatography, chemical crosslinking and analytical ultracentrifugation. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25446653 -- 4.7 Кб -- 09.04.2016
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23. Prions and chaperones: friends or foes?
... Prions and chaperones: friends or foes? ... This review highlights the modern perception of anomalous folding of the prion protein and the role of chaperones therein. Special attention is paid to prion proteins from mammalian species, which are prone to amyloid-like prion diseases due to a unique aggregation pathway. ... The most interesting of those are connected to the interaction with chaperone system, which is responsible for stabilizing protein structure and disrupting aggregates. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25365486 -- 4.5 Кб -- 09.04.2016
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24. Identification of a Mg2+-sensitive ORF in the 5'-leader of TRPM7 magnesium
... Identification of a Mg2+-sensitive ORF in the 5'-leader of TRPM7 magnesium channel mRNA. ... 2014 Nov 10;42(20):12779-88. doi: 10.1093/nar/gku951. Epub 2014 Oct 17. ... We found that TRPM7 mRNA 5'-leader contains two evolutionarily conserved upstream open reading frames that act together to drastically inhibit translation of the TRPM7 reading frame at high magnesium levels and ensure its optimal translation at low magnesium levels, when the activity of the channel-kinase is most required. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25326319 -- 4.0 Кб -- 09.04.2016
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25. Mitochondrial cytochrome c oxidase and control of energy metabolism:
... Mitochondrial cytochrome c oxidase and control of energy metabolism: measurements in suspensions of isolated mitochondria. ... Adding an uncoupler of oxidative phosphorylation eliminates the dependence of the cytochrome c oxidase activity on pH and energy state. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25324517 -- 4.8 Кб -- 09.04.2016
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26. phyA-GFP is spectroscopically and photochemically similar to phyA and comprises
... phyA-GFP is spectroscopically and photochemically similar to phyA and comprises both its native types, phyA' and phyA''. ... Thus, phyA-GFP is spectroscopically and photochemically similar or identical to the native phyA, suggesting that the GFP tag does not affect the chromophore. phyA-GFP comprises phyA'-GFP and phyA''-GFP, suggesting that both of them are potential participants in nuclear-cytoplasmic partitioning, which may contribute to its complexity. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25297540 -- 4.8 Кб -- 09.04.2016
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27. Characterization of human small heat shock protein HspB1 that carries
... Characterization of human small heat shock protein HspB1 that carries C-terminal domain mutations associated with hereditary motor neuron diseases. ... Mutation P182S induced the formation of metastable large oligomers of HspB1 with apparent molecular weight of more than 1000kDa. ... All mutations analyzed are associated with hereditary motor neuropathies or Charcot-Marie-Tooth disease type 2; however, molecular mechanisms underlying pathological effects are specific for each of these mutants. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25220807 -- 4.9 Кб -- 09.04.2016
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28. His166 is the Schiff base proton acceptor in attractant phototaxis receptor
... His166 is the Schiff base proton acceptor in attractant phototaxis receptor sensory rhodopsin I. Authors: . ... His166 on the cytoplasmic side of the SB in SRI has been implicated in the SB proton transfer reaction by mutation studies, and mutants of His166 result in an inverted SB proton release to the EC as well as inversion of the protein's normally attractant phototaxis signal to repellent. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25162914 -- 4.5 Кб -- 09.04.2016
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29. Regulation of glucokinase activity in liver of hibernating ground squirrel
... Regulation of glucokinase activity in liver of hibernating ground squirrel Spermophilus undulatus. ... The kinetic properties of glucokinase (GLK) from the liver of active and hibernating ground squirrels Spermophilus undulatus have been studied. Entrance of ground squirrels into hibernation from their active state is accompanied by a sharp decrease in blood glucose (Glc) level (from 14 to 2.9 mM) and with a significant (7-fold) decrease of GLK activity in the liver cytoplasm. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25108335 -- 5.0 Кб -- 09.04.2016
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30. Sperm-specific glyceraldehyde-3-phosphate dehydrogenase is stabilized by
... Sperm-specific glyceraldehyde-3-phosphate dehydrogenase is stabilized by additional proline residues and an interdomain salt bridge. ... Sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) exhibits enhanced stability compared to the somatic isoenzyme (GAPD). A comparative analysis of the structures of these isoenzymes revealed characteristic features, which could be important for the stability of GAPDS: six specific proline residues and three buried salt bridges. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25091199 -- 5.3 Кб -- 09.04.2016
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31. Protein interactomics based on direct molecular fishing on paramagnetic
... Protein interactomics based on direct molecular fishing on paramagnetic particles: practical realization and further SPR validation. ... There is increasing evidence that proteins function in the cell as integrated stable or temporally formed protein complexes, interactomes. Previously, using model systems we demonstrated applicability of direct molecular fishing on paramagnetic particles for protein interactomics (Ershov et al. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=25044858 -- 5.8 Кб -- 09.04.2016
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32. Creation of catalytically active particles from enzymes crosslinked with a
... Creation of catalytically active particles from enzymes crosslinked with a natural bifunctional agent--homocysteine thiolactone. ... The current study describes an approach to creation of catalytically active particles with increased stability from enzymes by N-homocysteinylation, a naturally presented protein modification. ... Modification of these proteins concerns the accessible lysine residues and introduces an average of 2-2,5 homocysteine residues per protein monomer. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=24912753 -- 5.2 Кб -- 09.04.2016
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33. Spectral and kinetic parameters of phosphorescence of triplet chlorophyll a in
... Spectral and kinetic parameters of phosphorescence of triplet chlorophyll a in the photosynthetic apparatus of plants. ... Spectral and kinetic parameters and quantum yield of IR phosphorescence accompanying radiative deactivation of the chlorophyll a (Chl a) triplet state were compared in pigment solutions, greening and mature plant leaves, isolated chloroplasts, and thalluses of macrophytic marine algae. ... This parameter is stable in leaves of different plant species. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=24910208 -- 5.1 Кб -- 09.04.2016
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34. Effect of disulfide crosslinking on thermal transitions and chaperone-like
... Effect of disulfide crosslinking on thermal transitions and chaperone-like activity of human small heat shock protein HspB1. ... Temperature-induced conformational changes of reduced and oxidized HspB1 crosslinked by disulfide bond between single Cys137 of neighboring monomers were analyzed by means of different techniques. ... Oxidation resulted in decrease of chaperone-like activity of HspB1. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=24898092 -- 4.5 Кб -- 09.04.2016
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35. Glutathionylation of the alpha-subunit of Na,K-ATPase from rat heart by
... Glutathionylation of the alpha-subunit of Na,K-ATPase from rat heart by oxidized glutathione inhibits the enzyme. ... A partially purified Na,K-ATPase preparation from rat heart containing alpha1- and alpha2-isoforms of the enzyme was shown to include both subunits in S-glutathionylated state. Glutathionylation of the alpha1-subunit (but not of the alpha2-subunit) was partially removed when the preparation was isolated in the presence of dithiothreitol. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=24794731 -- 4.0 Кб -- 09.04.2016
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36. Role of a helix B lysine residue in the photoactive site in channelrhodopsins.
... Role of a helix B lysine residue in the photoactive site in channelrhodopsins. ... In contrast, the corresponding neutralizing mutations in some relatively low-efficiency channelrhodopsins (ChRs) result in blue shifts. These ChRs do not contain a lysine residue in the second helix, conserved in higher efficiency ChRs (Lys-132 in the crystallized ChR chimera). ... In the three ChRs tested the Lys-132 homolog also alters channel current kinetics. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=24739160 -- 5.2 Кб -- 09.04.2016
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37. ATPase/synthase activity of Paracoccus denitrificans Fo.F1 as related to the
... ATPase/synthase activity of Paracoccus denitrificans Fo.F1 as related to the respiratory control phenomenon. ... The time course of ATP synthesis, oxygen consumption, and change in the membrane potential in Paracoccus denitrificans inside-out plasma membrane vesicles was traced. ... At pH7.0, no reversal of the ATP synthase reaction by the uncoupler was seen. ... Possible mechanisms of kinetic control and unidirectional operation of the Fo.F1-ATP synthase are discussed. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=24732246 -- 4.4 Кб -- 09.04.2016
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38. Silencing AML1-ETO gene expression leads to simultaneous activation of both
... Silencing AML1-ETO gene expression leads to simultaneous activation of both pro-apoptotic and proliferation signaling. ... A reduction in KIT mRNA levels was also observed in AE-silenced cells, but silencing KIT expression reduced cell growth but did not induce apoptosis. Transcription profiling of cells that escape cell death revealed activation of a number of signaling pathways involved in cell survival and proliferation. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=24727677 -- 5.3 Кб -- 09.04.2016
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39. Chaperone-like activity of monomeric human 14-3-3zeta on different protein
... Chaperone-like activity of monomeric human 14-3-3zeta on different protein substrates. ... Members of the 14-3-3 protein family interact with hundreds of different, predominantly phosphorylated, proteins. 14-3-3 dimers are prevalent but exist at the equilibrium with the monomers. ... Since the chaperone-like activity of 14-3-3 monomers has been insufficiently studied, here we have analyzed the effect of 14-3-3zetam on the aggregation of different model proteins. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=24681339 -- 4.6 Кб -- 09.04.2016
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40. lumi-R step.
... lumi-R step. ... 2014 Jul-Aug;90(4):786-95. doi: 10.1111/php.12263. Epub 2014 Mar 28. ... 24571438 . Abstract . Pfr from 0.13 in the wild type to 0.05-0.07 in the mutants. ... The critical role of the tyrosine hydroxyl group can be at the prelumi-R bifurcation point to optimize the yield of the photoprocess and energy storage in the form of lumi-R for subsequent rearrangement processes culminating in Pfr formation. ...
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Ссылки http://biochem.bio.msu.ru/publications/publication.php?pubmedID=24571438 -- 4.8 Кб -- 09.04.2016
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