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61. MSM 2007
... The recent data [1] suggested, that the calcium gradient might be one of the key factors involved in the regulation of the functional states of the Ca2+-ATPase and the Ca2+-channels of Ca2+-store sarcoplasmic reticulum (SR). ... The fluorescent probes pyrene and merocyanine 540 were introduced into the vesicles after loading with various concentrations of calcium (0 mM, 0.3 mM, 1mM, and 3 mM). The protein intrinsic tryptophan fluorescence was also used as a probe [2]. ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p15006/ -- 6.9 Кб -- 01.10.2012
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62. MSM 2007
... 495) 1419875, Fax: (495) 4150975, email: koltovoi@mail.ru . ... for medicine. ... Spectral range is 400-1000 . nm, region for spectral research is from 4 microm to 4mm. ... Linear and circular polarization for biopolymers. We used two types of polarizations: liner . and circular. All objects have different properties for different polarizations. ... Described optical properties of spherolites in biology, medicine and biopolymers. abstract (PDF) . 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p15002/ -- 4.7 Кб -- 01.10.2012
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63. MSM 2007
... Using Raman spectroscopy the changes in conformation of haemoglobin haemoporphyrin (HH) under haemoglobin (Hb) affinity to O2 modifications (acidic pH, 2,3-diphosphoglycerate (2,3-DPG)) was investigated. The typical Raman spectra of HH at pH 7.4 (dotted line) and pH 5 (solid line) is shown in figure A. We have shown that acidic pH and 2,3-DPG actions changes of HH conformation and decreases Hb affinity to oxygen. ... The HH conformation changes will discuss (fig. ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p15001/ -- 5.3 Кб -- 01.10.2012
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64. MSM 2007
... 10 years ago the combination of two technologies ? genomic that allows to sequence genes of biosystems and mass-spectrometry analysis - converted the protein biochemistry into the Proteomics. ... Due to PCR there is no detection limit in genomics. ... Molecular counting technology in combination with the technology that allows to fish up and concentrate protein molecules on the surface consisting of high specific immobilized antybodies, aptamers and other ligates are used. ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14998/ -- 6.3 Кб -- 01.10.2012
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65. MSM 2007
... We applied microscopy-pulse amplitude modulation chlorophyll fluorometer (Microscopy PAM, Walz, Effeltrich, Germany) combined with an Axiovert 25 CFL inverted microscope (Carl Zeiss, Germany) to assess photosynthesis at the subcellular level by chlorophyll fluorescence using the saturation pulse method. ... When the weak measuring light is switched on, the minimal fluorescence yield, Fo, is assessed which is characteristic for the dark-adapted state with all PSII reaction centers (RC) being open. ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14993/ -- 6.5 Кб -- 01.10.2012
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66. MSM 2007
... Osipov A.N. , Stepanov G.O. , Vladimirov Yu.A. 1 , Kagan V.E. 2 . ... One of the most important events in the development of apoptosis is the enhancement of cytochrome c peroxidase activity upon its interaction with cardiolipin, which modifies the active center of cytochrome c [3-5]. In the present work, the effects of nitric oxide on the cytochrome c peroxidase activity when cytochrome c is bound to cardiolipin have been investigated. ... 14, 4 (2007). ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14967/ -- 7.2 Кб -- 01.10.2012
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67. MSM 2007
... BIO-313, Biosystems Department Danish Polymer Centre, Risoe National Laboratory, Technical University of Denmark, DK-4000 Roskilde, Denmark . ... 2 Department of Physics, Stanford University, Stanford, CA 94305, USA . ... The limited number of photons available from a single fluorescent molecule makes optimal data analysis is as important to experimental resolution as improved experimental conditions, such as photo bleaching rates of fluorescent probes. ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14965/ -- 5.8 Кб -- 01.10.2012
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68. MSM 2007
... Mosekilde E. Department of Physics, Technical University of Denmark, Kgs. ... The purpose of this talk is to demonstrate the essential role that nonlinear dynamic phenomena play in normal physiological regulation and in the development of various diseases. ... Regulation of pressures and flows in the individual nephron involves the interaction between the tubuloglomerular feedback mechanism (TGF) and the oscillatory response of the activated afferent arteriole. ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14959/ -- 6.4 Кб -- 01.10.2012
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69. MSM 2007
... It was shown that approximately half of mercury injected into a human organism is bounded by red blood cells. In this work the influence of mercuric chloride (HgCl 2 ) on the rate of oxygen uptake and release by human red blood cells was investigated. ... Incubation of red blood cells (Hct 0,2% before mixing) with HgCl 2 causes essential decrease of the oxygen uptake and release rates. At concentration of 160 ?M HgCl 2 was shown to cause the 2.7 fold decrease of oxygen uptake rate. ... Res. ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14950/ -- 7.0 Кб -- 01.10.2012
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70. MSM 2007
... Quaternary structure of protein is an association when new biological properties appear which are unusual in tertiary structure. ... Catalytic activity was determined by spectrophotometric method with the use of rhodamine 6G, amount of protein was assayed by Lawry method. ... This method allows to obtain an image of immobilized on mica lipase surface, and this permits to conclude an important role of quaternary structure in lipase catalytic activity regulation. abstract (PDF) . ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14948/ -- 6.2 Кб -- 01.10.2012
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71. MSM 2007
... Applications of near-infrared spectroscopic (NIRS) imaging to detection, localization and grading of acute cardiac ischemia and chronic infarction in pig models in vivo are described. ... Kinetics of passage of NIR flow tracer, indocyanine green (ICG), imaged at 800 nm provided relative flow distribution. ... NIRS imaging is well suited for intra-operative applications during coronary artery bypass surgery in humans. ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14945/ -- 5.4 Кб -- 01.10.2012
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72. MSM 2007
... Several compounds of platinum, e.g. cisplatin and carboplatin, display strong cytostatic acitvity and are potent anticancer drugs, widely used in clinics. Extensive studies of mechanism of action of platinum drugs and platinum-induced DNA damage were held in last decades. ... Though platination of pure DNA is a well studied process, the relative rates of reactions with therapy target (DNA), and toxicity and side targets (proteins) are poorly studied, and many controversies remain. ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14943/ -- 6.8 Кб -- 01.10.2012
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73. MSM 2007
... Kingdon, Penning and Paul ion traps invented correspondingly in 1923, 1936 and 1956 have played essential role in 20th century physics- test of the validity of quantum electrodynamics, tests of symmetry properties of fundamental laws-particle-antiparticle (electron-positron), the electric dipole moment of the neutron, ion spectroscopy, hyromagnetic ratio, comparison of masses m(p+/m(e-) m(CO+)/m(N2+) frequency and time standards, dusty plasma and so on. ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14940/ -- 6.8 Кб -- 01.10.2012
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74. MSM 2007
... New insights into cytogenetics from simultaneous painting of all pairs of chromosomes . Multicolor in situ hybridization (m-FISH) and Spectral Karyotyping (SKY) are molecular cytogenetic techniques that permit the simultaneous visualization of all human (or mouse) chromosomes in different colours (chromosome painting), facilitating a detailed karyotype analysis. ... In the present talk new insights into the induction of aberrations by high + low LET radiation, analysed by m-FISH will be discussed. ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14880/ -- 6.6 Кб -- 01.10.2012
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75. MSM 2007
... By this assay we have studied the change of the expressional level of membrane-bound CD4-markers of native and modified T-lymphocytes from human blood. ?-interferon was used for modification T-cells. ... Moscow). ... The increase of interferon concentration up to 100 IU/ml decreased the values of optical density to 0.317?0.020, but with using 10 IU/ml concentration of modificator it was no significant differences from control level. ... 2007 Biophysics dep., Biological fac., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14872/ -- 6.2 Кб -- 01.10.2012
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76. MSM 2007
... Recently we described a novel method for forming substrate-supported macroscopically-aligned lipid bilayers by self-assembling phospholipids into nanotubular structures inside ordered nanochannels of anodic aluminum oxide (AAO) [1]. We have also shown that these nanotubular bilayers retain many properties of unsupported bilayers and are suitable for incorporating transmembrane peptides and small membrane proteins [2]. ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14869/ -- 6.7 Кб -- 01.10.2012
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77. MSM 2007
... Faculty of Physics, St. Petersburg State University, 1 Uljanovskaya st., Stary Petergof, Saint-Petersburg, 198504, Russian Federation, +7-(812)-428-4388, Polyanichko@gmail.com . ... St. Petersburg, 194064, Russian Federation, +7-(812)-297-3740 . A combination of ultraviolet (UV) and infrared (IR) absorption and circular dichroism (CD) spectroscopy was applied to investigate the structure and formation of large supramolecular DNA?protein complexes. ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14867/ -- 6.3 Кб -- 01.10.2012
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78. MSM 2007
... The cuvettes provide for many-fold increase of fluorescence intensity due to multiple passage of exciting light through the solution being tested and due to additional fluorescence collected. In the mirror cuvette, the exciting light is reflected by aluminium layer, applied at the outer walls of a quartz cell and provided with protecting coating. The exciting light entering through a narrow window in the center of the front mirror wall undergoes two or three reflections inside the cuvette. ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14866/ -- 5.7 Кб -- 01.10.2012
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79. MSM 2007
... intrinsic fluorescence, diffuse reflectance and NIR spectroscopic imaging ? are combined as related to a multimodal approach in cardiovascular research and diagnostics. ... Both Kubelka-Munk and photon migration (PM) approaches theoretically describe diffuse reflectance, reabsorption and emission tracks in the tissue and were employed to support the reabsorption effect. ... Diffuse reflectance of a beating heart either in vivo or ex-vivo is monochromatically collected by CCD camera. ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14861/ -- 6.7 Кб -- 01.10.2012
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80. MSM 2007
... We investigate influence of UV radiation on the photosynthetic apparatus of plants. ... Influence UV irradiation on kinetic of photoinduced of electronic transport and paramagnetic properties isolated chloroplasts and leaves of higher plants is investigated. ... It is observed that in the darkness irradiated leaves and isolated chloroplast change the form of dark signal EPR 2, caused by accumulation of new stable radicals with g ~ 2,004 is observed; ?Н = 8 Qs. ... 2007 Biophysics dep., ...
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Ссылки http://www.msm.biophys.msu.ru/eng/presentations/p14856/ -- 6.7 Кб -- 01.10.2012
[ Сохраненная копия ] Ссылки http://www.msm.biophys.msu.ru/presentations/p14856/ -- 6.7 Кб -- 01.10.2012
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