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81. http://www.genebee.msu.ru/LPSR/Pestova_MCB1996-1.pdf
... These processes are both mediated by eukaryotic initiation factor 4F (eIF4F), which consists of eIF4A (helicase), eIF4E (cap-binding protein), and eIF4G subunits. ... We have recently found that the initiation mediated by the IRES of encephalomyocarditis virus (EMCV) has the same requirements (45). eIF4A exhibits RNA-dependent ATPase activity and, in conjunction with eIF4B, RNA helicase activity (29, 49). ... 16, 1996 FUNCTIONAL DOMAINS OF eIF4G 6871 FIG. ... UV cross-linking of EMCV RNA. ... FIG. ...
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Ссылки http://www.genebee.msu.ru/LPSR/Pestova_MCB1996-1.pdf -- 425.1 Кб -- 01.12.2008
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82. http://www.genebee.msu.ru/LPSR/Ostareck_Cell2001.pdf
... 104, 281ґ290, January 26, 2001, Copyright ?2001 by Cell Press Lipoxygenase mRNA Silencing in Erythroid Differentiation: The 3 UTR Regulatory Complex Controls 60S Ribosomal Subunit Joining Dirk H. Ostareck,* Antje Ostareck-Lederer,* Ivan N. Shatsky, and Matthias W. Hentze* * Gene Expression Programme European Subsequently, the 5 UTR is "scanned" to identify the translation initiation codon to which the 43S complex repositions (Kozak, 1989). ... Cell 282 Figure 1. ...
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Ссылки http://www.genebee.msu.ru/LPSR/Ostareck_Cell2001.pdf -- 544.8 Кб -- 01.12.2008
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83. http://www.genebee.msu.ru/LPSR/Pestova_GnD1998.pdf
... Ribosomal subunits (40S) formed stable binary complexes on both mRNAs. ... We report here that we have reconstituted HCV and CSFV IRES-mediated initation in vitro up to the stage of 48S complex formation using purified components (Metґ tRNAMet, 40S subunits, initiation factors, and RNA) to i identify which factors are requred for this process and begin to characterize their functions during it. ... We used this method to analyze binding of mammalian 40S ribosomal subunits to HCV and CSFV IRESs. ...
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Ссылки http://www.genebee.msu.ru/LPSR/Pestova_GnD1998.pdf -- 1097.1 Кб -- 01.12.2008
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84. http://www.genebee.msu.ru/LPSR/Kolupaeva_JBC1998.pdf
... The site of ribosomal entry onto most mRNAs is determined by interaction of the 5 -terminal cap with eIF4E; eIFs 4A and 4G may facilitate ribosomal entry by modifying mRNA structure near the cap and by interacting with ribosome-associated factors. eIF4G recruits uncapped encephalomyocarditis virus (EMCV) mRNA to ribosomes without the involvement of eIF4E by binding directly to the 450-nucleotide long EMCV internal ribosome entry site (IRES). ... UV cross-linking of initiation factors to the EMCV...
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Ссылки http://www.genebee.msu.ru/LPSR/Kolupaeva_JBC1998.pdf -- 507.2 Кб -- 01.12.2008
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85. http://www.genebee.msu.ru/LPSR/Sizova_JVi1998.pdf
... 6 Specific Interaction of Eukaryotic Translation Initiation Factor 3 with the 5 Nontranslated Regions of Hepatitis C Virus and Classical Swine Fever Virus RNAs DARIA V. SIZOVA,1 VICTORIA G ... at Brooklyn, Brooklyn, New York 11203-20982 Received 9 October 1997/Accepted 12 February 1998 Translation of hepatitis C virus ( HCV ) and classical swine fever virus ( ... Transcription of HCV and CSFV RNAs. ... Simultaneous binding of eIF3 and 40S ribosomal subunits to CSFV and HCV IRES elements. ...
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Ссылки http://www.genebee.msu.ru/LPSR/Sizova_JVi1998.pdf -- 2641.8 Кб -- 01.12.2008
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86. http://www.genebee.msu.ru/LPSR/Pestova_PNAS2001.pdf
... Initiation on some EMCV-like IRESs requires additional noncanonical initiation factors, which alter IRES conformation and promote binding of eIF4A 4G. Initiation on the hepatitis C virus IRES is even simpler: 43S complexes containing only eIF2 and eIF3 bind directly ... initiation codon as a result of specific interaction of the IRES and the 40S subunit. (iv) Displacement of factors f rom the 48S c omplex and join ing of the 60S subun it to for m an 80S ribosome, leav ...
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Ссылки http://www.genebee.msu.ru/LPSR/Pestova_PNAS2001.pdf -- 135.5 Кб -- 01.12.2008
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87. http://www.genebee.msu.ru/LPSR/Pisarev_JBC2002.pdf
... 15445ґ15451, 2002 Printed in U.S.A. Positive and Negative Effects of the Major Mammalian Messenger Ribonucleoprotein p50 on Binding of 40 S Ribosomal Subunits to the Initiation Codon of - Globin mRNA * Received for publication, December 14, 2001, and in revised form, February 13, 2002 Published, JBC Papers in Press, February 19 ... Comparative study of effects of p50 and PTB on the formation of 48 S preinitiation complexes with -globin mRNA at different protein/mRNA ratios. ... In Fig. ... FIG...
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Ссылки http://www.genebee.msu.ru/LPSR/Pisarev_JBC2002.pdf -- 319.1 Кб -- 01.12.2008
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88. Лаборатория регуляции белкового синтеза
... О лаборатории . ... В 90-е годы основные усилия были направлены на изучение феномена внутренней инициации трансляции. мРНК некоторых вирусов человека и животных, жизненный цикл которых проходит целиком в цитоплазме клетки (пикорнавирусов, пестивирусов и некоторых других), не содержат на 5'-конце 7-метилгуанозинового кепа, который необходим всем эукариотическим мРНК для связывания факторов инициации трансляции и привлечения рибосомы. ... Так, в 2001 году совместно с лаб. ...
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Ссылки http://www.genebee.msu.ru/LPSR/about.html -- 9.7 Кб -- 30.11.2008
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89. Лаборатория регуляции белкового синтеза
... I.M. Terenin, S.E. Dmitriev, D.E. Andreev, I.N. Shatsky .( ... Biol. ... PubMed ][ PDF ] . ... Mol Biol (Mosk) . ... D.E. Andreev, I.M Terenin, S.E. Dmitriev, I.N. Shatskii (2006) Similar features in mechanisms of translation initiation of mRNAs in eukaryotic and prokaryotic systems. ... D.E. Andreev, I.M. Terenin, Y.E. Dunaevsky, S.E. Dmitriev, I.N. Shatsky (2006) A leaderless mRNA c an bind to mammalian 80S ribosomes and direct polypeptide synthesis in the absence of translation initiation factors...
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Ссылки http://www.genebee.msu.ru/LPSR/ENG/allpublications.html -- 37.9 Кб -- 30.11.2008
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90. http://www.genebee.msu.ru/LPSR/ENG/Dmitrev_MolBiol2006Eng.pdf
... PRIORITY COMMUNICATIONS UDC 577.217.52 Adequate System for Studying Translation Initiation on the Human Retrotransposon L1 mRNA in Vitro S. E. Dmitriev, N. V. Bykova, D. E. Andreev, and I. M. Terenin Belozersky Institute of Physico-Chemical Biology, Moscow ... When RRL was supplemented with a cytoplasmic extract of HeLa cells, the aberrant products were not synthesized, while the first cistron was translated with the same efficiency. ... Here and in Fig. 2, p40ab are aberrant products. ...
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Ссылки http://www.genebee.msu.ru/LPSR/ENG/Dmitrev_MolBiol2006Eng.pdf -- 146.5 Кб -- 30.11.2008
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91. http://www.genebee.msu.ru/LPSR/ENG/Andreev_MolBiol2006Eng.pdf
... UDC 577.213:577.217 Similar Features in the Mechanisms of mRNA Translation Initiation in Eukaryotic and Prokaryotic Systems D. E. Andreev, I. M. Terenin, S. E. Dmitriev, and I. N. Shatsky Belozersky Institute of Physicochemical ... 119992 Russia; e-mail: shatsky@libro.genebee.msu.su Received February 20, 2006 Abstract--Similar features in the mechanisms of mRNA translation initiation on prokaryotic and ... Circularization of mRNA by eukaryotic translation initiation factors. ...
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Ссылки http://www.genebee.msu.ru/LPSR/ENG/Andreev_MolBiol2006Eng.pdf -- 191.8 Кб -- 30.11.2008
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92. http://www.genebee.msu.ru/LPSR/ENG/Pisarev_MolBiol2004Eng.pdf
... Translated from Molekulyarnaya Biologiya, Vol. ... MOLECULAR MECHANISMS OF BIOLOGICAL PROCESSES UDC 577.217.52 Mutual Effects of Translation Initiation Factors in Binding to the IRES of the Encephalomyocarditis Virus RNA A. V. Pisarev, S. E. Dmitriev, and I. N. Shatsky Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119899 Russia E-mail: shatsky@libro.genebee.msu.su Received July 7, 2003 Key words: translation initiation , IRES , initiation ... RNA. ...
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Ссылки http://www.genebee.msu.ru/LPSR/ENG/Pisarev_MolBiol2004Eng.pdf -- 295.2 Кб -- 30.11.2008
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93. http://www.genebee.msu.ru/LPSR/ENG/Dmitriev_MolBol2003Eng.pdf
... MOLECULAR MECHANISMS OF BIOLOGICAL PROCESSES UDC 577.217.52 Minor Secondary - Structure Variation in the 5'-Untranslated Region of the b-Globin mRNA Changes the Concentration Requirements for eIF2 S. E. Dmitriev, I. M. Terenin, M. P. Rubtsova, and I. N. Shatsky Belozerskii Institute of Physico-Chemical Biology, Moscow State ... Received September 24, 2002 Abstract--Nucleotide sequence changes increasing the number of paired bases without producing stable secondary ...
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Ссылки http://www.genebee.msu.ru/LPSR/ENG/Dmitriev_MolBol2003Eng.pdf -- 140.4 Кб -- 30.11.2008
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94. http://www.genebee.msu.ru/LPSR/ENG/Olovnikov_MolBiol2007Eng.pdf
... PROTEOMICS UDC 577.217.52 Priority publication Key Role of the Internal 5'- UTR Segment in the Transcription Activity of the Human L1 Retrotransposon I. A. Olovnikova, Z. V. Adyanovab, E. R. Galimova, D. E. Andreeva, I. M. Terenina, D. S. Ivanovb, V. S. Prassolovb ... Received and accepted for publication November 17, 2006 Presented for publication by I.N. Shatsky Abstract--The long 5'-untranslated region (5'- UTR ) of the ... Expression of Arabidopsis LINEs from two promoters. ...
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Ссылки http://www.genebee.msu.ru/LPSR/ENG/Olovnikov_MolBiol2007Eng.pdf -- 185.7 Кб -- 30.11.2008
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95. Лаборатория регуляции белкового синтеза
... I.M. Terenin, S.E. Dmitriev, D.E. Andreev, I.N. Shatsky .( ... PubMed ][ PDF ] . ... D.E. Andreev, I.M. Terenin, Y.E. Dunaevsky, S.E. Dmitriev, I.N. Shatsky (2006) A leaderless mRNA c an bind to mammalian 80S ribosomes and direct polypeptide synthesis in the absence of translation initiation factors. ... I.M. Terenin, S.E. Dmitriev, D.E. Andreev, E. Royall, G.J. Belsham, L.O. Roberts, I.N. Shatsky (2005) A cross-kingdom internal ribosome entry site reveals a simplified mode of internal ribosome...
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Ссылки http://www.genebee.msu.ru/LPSR/allpublications.html -- 23.6 Кб -- 30.11.2008
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96. http://www.genebee.msu.ru/LPSR/Laletina_NAR2006.pdf
... 34, No. 7 2027ґ2036 doi:10.1093/nar/gkl155 Proteins surrounding hairpin IIIe of the hepatitis C virus internal ribosome entry site on the human 40S ribosomal subunit Elena Laletina, Dmitri Graifer, Alexey Malygin, Anton Ivanov, Ivan Shatsky1 and Galina Karpova* Institute of Chemical Biology and ... Received January 26, 2006; Revised February 14, 2006; Accepted March 20, 2006 ABSTRACT Binding of the internal ribosome entry site ( IRES ) of the hepatitis C virus ( ... Ribosomal complexes. ...
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Ссылки http://www.genebee.msu.ru/LPSR/Laletina_NAR2006.pdf -- 332.8 Кб -- 30.11.2008
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97. http://www.genebee.msu.ru/LPSR/Pisarev_JVi2004.pdf
... In toeprinting assays, assembly of 48S preinitiation complexes from purified components on the PTV-1 IRES was Met achieved with just 40S ribosomal subunits plus eIF2 and Met-tRNAi . ... Comparison between the PTV-1 IRES and HCV IRES elements revealed islands of high sequence identity that occur in regions critical for the interactions of the HCV IRES with the 40S ribosomal subunit and eIF3. ... FIG. ... Sequence and structural similarity between the PTV-1 IRES and the HCV IRES. ... RNA 7:194ґ206. ...
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Ссылки http://www.genebee.msu.ru/LPSR/Pisarev_JVi2004.pdf -- 902.3 Кб -- 30.11.2008
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98. http://www.genebee.msu.ru/LPSR/Zolotukhin_MCB2003.pdf
... This downregulation can be counteracted by the viral Rev protein, resulting in efficient export and expression of these mRNAs. Here, we show that the INS region in HIV-1 gag mRNA is a high-affinity ligand of p54nrb/PSF, a heterodimeric transcription/splicing factor. ... 23, 2003 DOWNREGULATION OF HIV-1 EXPRESSION BY PSF 6621 FIG. 2. p54nrb and PSF bind specifically to INS-RNA in vitro. ... To this end, we studied the effects of exogenous p54nrb and PSF on INS-mRNA expression in 293 cells (Fig. ...
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Ссылки http://www.genebee.msu.ru/LPSR/Zolotukhin_MCB2003.pdf -- 481.3 Кб -- 30.11.2008
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99. http://www.genebee.msu.ru/LPSR/Dmitriev_FEBS2003.pdf
FEBS 26856 FEBS Letters 533 (2003) 99^104 Conversion of 48S translation preinitiation complexes into 80S initiation complexes as revealed by toeprinting Sergey E. Dmitriev, Andrey V. Pisarev, Maria P. Rubtsova, Yan E. Dunaevsky, Ivan N. Shatsky Belozersky ... accepted 27 November 2002 First published online 6 December 2002 Edited by Horst Feldmann Abstract A method of analysis of translation initiation ... The formation of 80S complexes with L-globin mRNA has also been performed [15]. ...
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Ссылки http://www.genebee.msu.ru/LPSR/Dmitriev_FEBS2003.pdf -- 220.1 Кб -- 30.11.2008
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100. http://www.genebee.msu.ru/LPSR/Pisarev_MolBiol2004.pdf
хщужфьу?ъчФ? ... Ч ЬвкгflТТ ,ТЯfl ЫС,ТвкЬА кЫ гвЬг,ЬАi длкЫ в, flСА ,ЬЫfl Яъчф в 40S влЗ~вкЫ^ТШ ЫЗгвгЯ л лЭЫгк: ЭЮввЫ~ТвЭЫШ ЭТд-С,ЫвЫЯАШ длкИ, ?лЬкЫлЫШ ЯТiЬЫСЯ Ы IRES-С,ЫвЫЯАШ вдгвгЗ ЫЬЫ ^Ы^ЫЫ кЬвЮfl^ЫЫ [вЯ. 1]. шЫ IRES-С,ЫвЫЯгЯ вдгвгЗТ, 40S влЗ~вкЫ^ в, flСА ,Тквfl ЬТ в 5'-ЭгЬ^гЯ Яъчф, вг вдТ^ЫйЫ~ТвЭЫЯ ,ЬлкТЬЬЫЯ л~вкЭгЯ ТТ 5'-чэщ (Internal Ribosome Entry Site, IRES). фЮввЫ~ТвЭЫШ дЫЯТ IRES-ЮТЯТЬкг, дТвк,Юflк вгЗгШ вдТ^ЫйЫ~ТвЭЫТ л~вкЭЫ в, flСА ,ЬЫfl ЫЗгвгЯ ... ХрщущЩр? кгЯ 38 < 2 2004 PTB 316 шЫвТ, Ы . ...
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Ссылки http://www.genebee.msu.ru/LPSR/Pisarev_MolBiol2004.pdf -- 87.7 Кб -- 30.11.2008
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